Figure 1. ATR3 belongs to the clade of NR1/Fre1 diflavin reductases. (a) Phylogenetic tree with per cent bootstrap value (1000 bootstrap) of 30 cytochrome P450 reductase (CPR) sequences and 12 ATR3-like sequences from 25 plant (in bold) and animal species. Aa, Artemisia annua; At, Arabidopsis thaliana; Ce, Caenorhabditis elegans; Cery, Centaurium erythraea; Chlamy, Chlamydomonas reinhardtii; Cr, Catharanthus roseus; Dm, Drosophila melanogaster; Gm, Glycine max; Hs, Homo sapiens; Ht, Helianthus tuberosus; Mm, Mus musculus; Mt, Medicago truncatula; Os, Oryza sativa; Ostta, Ostreococcus tauri; Pc, Petroselinum crispum; Pm, Pseudotsuga menziesii; Ps, Pisum sativum; Pso, Papaver somniferum; Ptd, Populus balsamifera subsp. trichocarpa × Populus deltoids; Rn, Rattus norvegicus; Sc, Saccharomyces cerevisiae; Ta, Triticum aestivum; Tc, Taxus chinensis; Vr, Vigna radiata; Vs, Vicia sativa. (b) Sequence alignment for FMN, FAD and NADPH cofactor-binding regions of consensus of plant/animal CPRs and plant/animal ATR3s. The protein consensus was obtained retaining only sequences from genome-sequencing projects ensuring that both CPR and ATR3 were available. Comprehensive alignment for all sequences is presented as in the Supporting Information, Fig. S1. Identical and similar residues are respectively black and grey box-shaded. The percentage of identity was calculated for each binding region and × indicates in the table which consensus sequences were compared. Asterisks indicate Ser457, Asp675 and Cys630, residues of the catalytic triad in CPRs.
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