The Arabidopsis LSD1 gene plays an important role in the regulation of low temperature-dependent cell death
Article first published online: 28 APR 2010
© The Authors (2010). Journal compilation © New Phytologist Trust (2010)
Volume 187, Issue 2, pages 301–312, July 2010
How to Cite
Huang, X., Li, Y., Zhang, X., Zuo, J. and Yang, S. (2010), The Arabidopsis LSD1 gene plays an important role in the regulation of low temperature-dependent cell death. New Phytologist, 187: 301–312. doi: 10.1111/j.1469-8137.2010.03275.x
- Issue published online: 24 JUN 2010
- Article first published online: 28 APR 2010
- Received: 23 January 2010, Accepted: 9 March 2010
- cell death;
- low temperature;
- reactive oxygen species (ROS)
- •In higher plants, the crosstalk between cold stress responses and reactive oxygen species (ROS) signaling is not well understood.
- •Two chilling-sensitive mutants, chs4-1 and chs4-3, were characterized genetically and molecularly.
- •The CHS4 gene, identified by map-based cloning, was found to be identical to LESION SIMULATING DISEASE RESISTANCE 1 (LSD1). We therefore renamed these two alleles lsd1-3 and lsd1-4, respectively. These two mutants exhibited an extensive cell death phenotype under cold stress conditions. Consistently, lsd1-3 plants exposed to cold showed up-regulation of the PR1 and PR2 genes, and increased accumulation of salicylic acid. These results indicate that low temperature is another trigger of cell death in lsd1 mutants. Furthermore, lsd1-3 plants accumulated higher concentrations of H2O2 and total glutathione under cold conditions than wild-type plants. Genetic analysis revealed that PAD4 and EDS1, two key signaling regulators mediating resistance responses, are required for the chilling-sensitive phenotype of lsd1-3.
- •These findings reveal a role of LSD1 in regulating cell death trigged by cold stress and a link between cold stress responses and ROS-associated signaling.