Genome-wide gene expression changes in genetically stable synthetic and natural wheat allohexaploids

Authors

  • Véronique Chagué,

    1. Organization and Evolution of Plant Genomes (OEPG), Unité de Recherche en Génomique Végétale (URGV), UMR INRA 1165 – CNRS 8114 – UEVE, F–91057 Evry Cedex, France
    Search for more papers by this author
  • Jérémy Just,

    1. Organization and Evolution of Plant Genomes (OEPG), Unité de Recherche en Génomique Végétale (URGV), UMR INRA 1165 – CNRS 8114 – UEVE, F–91057 Evry Cedex, France
    Search for more papers by this author
  • Imen Mestiri,

    1. Organization and Evolution of Plant Genomes (OEPG), Unité de Recherche en Génomique Végétale (URGV), UMR INRA 1165 – CNRS 8114 – UEVE, F–91057 Evry Cedex, France
    Search for more papers by this author
  • Sandrine Balzergue,

    1. Organization and Evolution of Plant Genomes (OEPG), Unité de Recherche en Génomique Végétale (URGV), UMR INRA 1165 – CNRS 8114 – UEVE, F–91057 Evry Cedex, France
    Search for more papers by this author
  • Anne-Marie Tanguy,

    1. Unité Mixte de Recherches INRA – Agrocampus Rennes, Amélioration des Plantes & Biotechnologies Végétales, F–35653 Le Rheu, France
    Search for more papers by this author
  • Cecile Huneau,

    1. Organization and Evolution of Plant Genomes (OEPG), Unité de Recherche en Génomique Végétale (URGV), UMR INRA 1165 – CNRS 8114 – UEVE, F–91057 Evry Cedex, France
    Search for more papers by this author
  • Virginie Huteau,

    1. Unité Mixte de Recherches INRA – Agrocampus Rennes, Amélioration des Plantes & Biotechnologies Végétales, F–35653 Le Rheu, France
    Search for more papers by this author
  • Harry Belcram,

    1. Organization and Evolution of Plant Genomes (OEPG), Unité de Recherche en Génomique Végétale (URGV), UMR INRA 1165 – CNRS 8114 – UEVE, F–91057 Evry Cedex, France
    Search for more papers by this author
  • Olivier Coriton,

    1. Unité Mixte de Recherches INRA – Agrocampus Rennes, Amélioration des Plantes & Biotechnologies Végétales, F–35653 Le Rheu, France
    Search for more papers by this author
  • Joseph Jahier,

    1. Unité Mixte de Recherches INRA – Agrocampus Rennes, Amélioration des Plantes & Biotechnologies Végétales, F–35653 Le Rheu, France
    Search for more papers by this author
  • Boulos Chalhoub

    1. Organization and Evolution of Plant Genomes (OEPG), Unité de Recherche en Génomique Végétale (URGV), UMR INRA 1165 – CNRS 8114 – UEVE, F–91057 Evry Cedex, France
    Search for more papers by this author

Author for correspondence:
Boulos Chalhoub
Tel: +33 1 60874503
Email: chalhoub@evry.inra.fr

Summary

  • The present study aims to understand regulation of gene expression in synthetic and natural wheat (Triticum aestivum) allohexaploids, that combines the AB genome of Triticum turgidum and the D genome of Aegilops tauschii; and which we have recently characterized as genetically stable.
  • We conducted a comprehensive genome-wide analysis of gene expression that allowed characterization of the effect of variability of the D genome progenitor, the intergenerational stability as well as the comparison with natural wheat allohexaploid. We used the Affymetrix GeneChip Wheat Genome Array, on which 55 049 transcripts are represented.
  • Additive expression was shown to represent the majority of expression regulation in the synthetic allohexaploids, where expression for more than c. 93% of transcripts was equal to the mid-parent value measured from a mixture of parental RNA. This leaves c. 2000 (c. 7%) transcripts, in which expression was nonadditive. No global gene expression bias or dominance towards any of the progenitor genomes was observed whereas high intergenerational stability and low effect of the D genome progenitor variability were revealed.
  • Our study suggests that gene expression regulation in wheat allohexaploids is established early upon allohexaploidization and highly conserved over generations, as demonstrated by the high similarity of expression with natural wheat allohexaploids.

Ancillary