Suppression of the AvrBs1-specific hypersensitive response by the YopJ effector homolog AvrBsT from Xanthomonas depends on a SNF1-related kinase
Article first published online: 1 JUL 2010
© The Authors (2010). Journal compilation © New Phytologist Trust (2010)
Special Issue: Featured papers on ‘Effectors in plant–microbe interactions’
Volume 187, Issue 4, pages 1058–1074, September 2010
How to Cite
Szczesny, R., Büttner, D., Escolar, L., Schulze, S., Seiferth, A. and Bonas, U. (2010), Suppression of the AvrBs1-specific hypersensitive response by the YopJ effector homolog AvrBsT from Xanthomonas depends on a SNF1-related kinase. New Phytologist, 187: 1058–1074. doi: 10.1111/j.1469-8137.2010.03346.x
- Issue published online: 12 AUG 2010
- Article first published online: 1 JUL 2010
- Received: 29 April 2010, Accepted: 18 May 2010
- bacterial spot disease;
- •Pathogenicity of the Gram-negative plant pathogen Xanthomonas campestris pv. vesicatoria (Xcv) depends on a type III secretion system that translocates a cocktail of > 25 type III effector proteins into the plant cell.
- •In this study, we identified the effector AvrBsT as a suppressor of specific plant defense. AvrBsT belongs to the YopJ/AvrRxv protein family, members of which are predicted to act as proteases and/or acetyltransferases.
- •AvrBsT suppresses the hypersensitive response (HR) that is elicited by the effector protein AvrBs1 from Xcv in resistant pepper plants. HR suppression occurs inside the plant cell and depends on a conserved predicted catalytic residue of AvrBsT. Yeast two-hybrid based analyses identified plant interaction partners of AvrBs1 and AvrBsT, including a putative regulator of sugar metabolism, SNF1-related kinase 1 (SnRK1), as interactor of AvrBsT. Intriguingly, gene silencing experiments revealed that SnRK1 is required for the induction of the AvrBs1-specific HR.
- •We therefore speculate that SnRK1 is involved in the AvrBsT-mediated suppression of the AvrBs1-specific HR.