Aluminium ions inhibit the formation of diacylglycerol generated by phosphatidylcholine-hydrolysing phospholipase C in tobacco cells

Authors

  • Přemysl Pejchar,

    1. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, v. v. i., Rozvojová 263, 165 02 Prague 6, Czech Republic
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  • Martin Potocký,

    1. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, v. v. i., Rozvojová 263, 165 02 Prague 6, Czech Republic
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  • Zuzana Novotná,

    1. Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Technická 5, 166 28 Prague 6, Czech Republic
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  • Štěpánka Veselková,

    1. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, v. v. i., Rozvojová 263, 165 02 Prague 6, Czech Republic
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  • Daniela Kocourková,

    1. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, v. v. i., Rozvojová 263, 165 02 Prague 6, Czech Republic
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  • Olga Valentová,

    1. Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Technická 5, 166 28 Prague 6, Czech Republic
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  • Kateřina Schwarzerová,

    1. Department of Experimental Plant Biology, Faculty of Science, Charles University in Prague, Viničná 5, 128 44 Prague 2, Czech Republic
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  • Jan Martinec

    1. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, v. v. i., Rozvojová 263, 165 02 Prague 6, Czech Republic
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Author for correspondence:
Jan Martinec
Tel: +420 225106416
Email: martinec@ueb.cas.cz

Summary

  • Aluminium ions (Al) have been recognized as a major toxic factor for crop production in acidic soils. This study aimed to assess the impact of Al on the activity of phosphatidylcholine-hydrolysing phospholipase C (PC-PLC), a new member of the plant phospholipase family.
  • We labelled the tobacco cell line BY-2 and pollen tubes with a fluorescent derivative of phosphatidylcholine and assayed for patterns of fluorescently labelled products. Growth of pollen tubes was analysed.
  • We observed a significant decrease of labelled diacylglycerol (DAG) in cells treated with AlCl3. Investigation of possible metabolic pathways that control DAG generation and consumption during the response to Al showed that DAG originated from the reaction catalysed by PC-PLC. The growth of pollen tubes was retarded in the presence of Al and this effect was accompanied by the decrease of labelled DAG similar to the case of the BY-2 cell line. The growth of pollen tubes arrested by Al was rescued by externally added DAG.
  • Our observation strongly supports the role of DAG generated by PC-PLC in the response of tobacco cells to Al.

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