Transcriptome profiles of hybrid poplar (Populus trichocarpa × deltoides) reveal rapid changes in undamaged, systemic sink leaves after simulated feeding by forest tent caterpillar (Malacosoma disstria)
Version of Record online: 29 JUL 2010
© The Authors (2010). Journal compilation © New Phytologist Trust (2010)
Volume 188, Issue 3, pages 787–802, November 2010
How to Cite
Philippe, R. N., Ralph, S. G., Mansfield, S. D. and Bohlmann, J. (2010), Transcriptome profiles of hybrid poplar (Populus trichocarpa × deltoides) reveal rapid changes in undamaged, systemic sink leaves after simulated feeding by forest tent caterpillar (Malacosoma disstria). New Phytologist, 188: 787–802. doi: 10.1111/j.1469-8137.2010.03392.x
- Issue online: 29 JUL 2010
- Version of Record online: 29 JUL 2010
- Received: 9 April 2010Accepted: 14 June 2010
Fig. S1 Schematic of microarray hybridization design.
Fig. S2 Comparison of variance and response obtained by microarray hybridizations using samples from individual trees separately or as pooled samples.
Fig. S3 Overall spatial and temporal patterns of differentially expressed (DE) genes in local and systemic leaves in response to forest tent caterpillar (FTC) oral secretions (OS) detected by transcriptome analysis on a microarray platform of 15 496 cDNA elements.
Fig. S4 Amino acid sequence alignment of galactinol synthases.
Table S1 Complete set of systemic microarray experiment results
Table S2 Oligonucleotide primers used in quantitative real-time PCR analyses
Table S3 Quantitative real-time PCR analysis for validation of microarray results
Table S4 Clustering analysis of microarray results
Table S5 Amino acid sequence relatedness of plant galactinol synthases
Table S6 Quantitative real-time PCR analysis of poplar galactinol synthase systemic expression
Methods S1 Microarray analysis and quantitative real-time PCR validation.
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