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Fig. S1 Schematic of microarray hybridization design.

Fig. S2 Comparison of variance and response obtained by microarray hybridizations using samples from individual trees separately or as pooled samples.

Fig. S3 Overall spatial and temporal patterns of differentially expressed (DE) genes in local and systemic leaves in response to forest tent caterpillar (FTC) oral secretions (OS) detected by transcriptome analysis on a microarray platform of 15 496 cDNA elements.

Fig. S4 Amino acid sequence alignment of galactinol synthases.

Table S1 Complete set of systemic microarray experiment results

Table S2 Oligonucleotide primers used in quantitative real-time PCR analyses

Table S3 Quantitative real-time PCR  analysis for validation of microarray results

Table S4 Clustering analysis of microarray results

Table S5 Amino acid sequence relatedness of plant galactinol synthases

Table S6 Quantitative real-time PCR analysis of poplar galactinol synthase systemic expression

Methods S1 Microarray analysis and quantitative real-time PCR validation.

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NPH_3392_sm_fS2.doc151KSupporting info item
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NPH_3392_sm_Legends.doc53KSupporting info item
NPH_3392_sm_tS1.xls16518KSupporting info item
NPH_3392_sm_tS2.doc36KSupporting info item
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NPH_3392_sm_tS4.xls6687KSupporting info item
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NPH_3392_sm_tS6.doc45KSupporting info item