A role for pectin de-methylesterification in a developmentally regulated growth acceleration in dark-grown Arabidopsis hypocotyls

Authors

  • Sandra Pelletier,

    1. Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech, INRA Centre de Versailles-Grignon, Route de St-Cyr (RD10), F–78026 Versailles Cedex, France
    2. Unité Mixte de Recherche de Genomique Végétale, Institut National pour la Recherche Agronomique/Centre National pour la Recherche Scientifique, 2 rue Gaston Crémieux-CP 5708. F–91057 Évry Cedex, France
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    • These authors contributed equally to this work.

  • Jürgen Van Orden,

    1. Plant Growth and Development, Biology Department, University of Antwerp, Groenenborgerlaan 171, BE–2020 Antwerpen, Belgium
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    • These authors contributed equally to this work.

  • Sebastian Wolf,

    1. Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech, INRA Centre de Versailles-Grignon, Route de St-Cyr (RD10), F–78026 Versailles Cedex, France
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  • Kris Vissenberg,

    1. Plant Growth and Development, Biology Department, University of Antwerp, Groenenborgerlaan 171, BE–2020 Antwerpen, Belgium
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  • Julien Delacourt,

    1. Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech, INRA Centre de Versailles-Grignon, Route de St-Cyr (RD10), F–78026 Versailles Cedex, France
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  • Yves Assoumou Ndong,

    1. Université de Picardie-Jules Verne, Unité de Recherche EA3900-BioPI, Biologie des Plantes et Contrôle des Insectes Ravageurs, Faculté des Sciences, Ilôt des Poulies, 33, Rue St Leu, F–80039 Amiens, France
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  • Jérôme Pelloux,

    1. Université de Picardie-Jules Verne, Unité de Recherche EA3900-BioPI, Biologie des Plantes et Contrôle des Insectes Ravageurs, Faculté des Sciences, Ilôt des Poulies, 33, Rue St Leu, F–80039 Amiens, France
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  • Volker Bischoff,

    1. Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech, INRA Centre de Versailles-Grignon, Route de St-Cyr (RD10), F–78026 Versailles Cedex, France
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  • Aurélie Urbain,

    1. Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech, INRA Centre de Versailles-Grignon, Route de St-Cyr (RD10), F–78026 Versailles Cedex, France
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  • Grégory Mouille,

    1. Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech, INRA Centre de Versailles-Grignon, Route de St-Cyr (RD10), F–78026 Versailles Cedex, France
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  • Gaëtan Lemonnier,

    1. Unité Mixte de Recherche de Genomique Végétale, Institut National pour la Recherche Agronomique/Centre National pour la Recherche Scientifique, 2 rue Gaston Crémieux-CP 5708. F–91057 Évry Cedex, France
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  • Jean-Pierre Renou,

    1. Unité Mixte de Recherche de Genomique Végétale, Institut National pour la Recherche Agronomique/Centre National pour la Recherche Scientifique, 2 rue Gaston Crémieux-CP 5708. F–91057 Évry Cedex, France
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  • Herman Höfte

    1. Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech, INRA Centre de Versailles-Grignon, Route de St-Cyr (RD10), F–78026 Versailles Cedex, France
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Author for correspondence:
Herman Höfte
Tel: +33 1 30833390
Email: herman.hofte@versailles.inra.fr

Summary

  • We focused on a developmentally regulated growth acceleration in the dark-grown Arabidopsis hypocotyl to study the role of changes in cell wall metabolism in the control of cell elongation.
  • To this end, precise transcriptome analysis on dissected dark-grown hypocotyls, Fourier transform infrared (FT-IR) microspectroscopy and kinematic analysis were used.
  • Using a cellulose synthesis inhibitor, we showed that the growth acceleration marks a developmental transition during which growth becomes uncoupled from cellulose synthesis. We next investigated the cellular changes that take place during this transition. FT-IR microspectroscopy revealed significant changes in cell wall composition during, but not after, the growth acceleration. Transcriptome analysis suggested a role for cell wall remodeling, in particular pectin modification, in this growth acceleration. This was confirmed by the overexpression of a pectin methylesterase inhibitor, which caused a delay in the growth acceleration.
  • This study shows that the acceleration of cell elongation marks a developmental transition in dark-grown hypocotyl cells and supports a role for pectin de-methylesterification in the timing of this event.

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