These authors contributed equally to this work.
A role for pectin de-methylesterification in a developmentally regulated growth acceleration in dark-grown Arabidopsis hypocotyls
Article first published online: 3 SEP 2010
© The Authors (2010). Journal compilation © New Phytologist Trust (2010)
Volume 188, Issue 3, pages 726–739, November 2010
How to Cite
Pelletier, S., Van Orden, J., Wolf, S., Vissenberg, K., Delacourt, J., Ndong, Y. A., Pelloux, J., Bischoff, V., Urbain, A., Mouille, G., Lemonnier, G., Renou, J.-P. and Höfte, H. (2010), A role for pectin de-methylesterification in a developmentally regulated growth acceleration in dark-grown Arabidopsis hypocotyls. New Phytologist, 188: 726–739. doi: 10.1111/j.1469-8137.2010.03409.x
- Issue published online: 19 OCT 2010
- Article first published online: 3 SEP 2010
- Received: 14 May 2010Accepted: 20 June 2010
- Arabidopsis thaliana;
- cell elongation;
- cell wall;
- Fourier transform infrared (FT-IR) microspectroscopy;
- pectin de-methylesterification;
- •We focused on a developmentally regulated growth acceleration in the dark-grown Arabidopsis hypocotyl to study the role of changes in cell wall metabolism in the control of cell elongation.
- •To this end, precise transcriptome analysis on dissected dark-grown hypocotyls, Fourier transform infrared (FT-IR) microspectroscopy and kinematic analysis were used.
- •Using a cellulose synthesis inhibitor, we showed that the growth acceleration marks a developmental transition during which growth becomes uncoupled from cellulose synthesis. We next investigated the cellular changes that take place during this transition. FT-IR microspectroscopy revealed significant changes in cell wall composition during, but not after, the growth acceleration. Transcriptome analysis suggested a role for cell wall remodeling, in particular pectin modification, in this growth acceleration. This was confirmed by the overexpression of a pectin methylesterase inhibitor, which caused a delay in the growth acceleration.
- •This study shows that the acceleration of cell elongation marks a developmental transition in dark-grown hypocotyl cells and supports a role for pectin de-methylesterification in the timing of this event.