Hypoxia responsive gene expression is mediated by various subsets of transcription factors and miRNAs that are determined by the actual oxygen availability
Article first published online: 14 SEP 2010
© The Authors (2010). Journal compilation © New Phytologist Trust (2010)
Special Issue: Plant anaerobiosis
Volume 190, Issue 2, pages 442–456, April 2011
How to Cite
Licausi, F., Weits, D. A., Pant, B. D., Scheible, W.-R., Geigenberger, P. and van Dongen, J. T. (2011), Hypoxia responsive gene expression is mediated by various subsets of transcription factors and miRNAs that are determined by the actual oxygen availability. New Phytologist, 190: 442–456. doi: 10.1111/j.1469-8137.2010.03451.x
- Issue published online: 28 MAR 2011
- Article first published online: 14 SEP 2010
- Received: 11 June 2010, Accepted: 22 July 2010
- microRNA (miRNA);
- quantitative real-time PCR (qRT-PCR);
- transcription factor
- •Reduced oxygen availability is not only associated with flooding, but occurs also during growth and development. It is largely unknown how hypoxia is perceived and what signaling cascade is involved in activating adaptive responses.
- •We analysed the expression of over 1900 transcription factors (TFs) and 180 microRNA primary transcripts (pri-miRNAs) in Arabidopsis roots exposed to different hypoxic conditions by means of quantitative PCR. We also analysed the promoters of genes induced by hypoxia with respect to over-represented DNA elements that can act as potential TF binding sites and their in vivo interaction was verified.
- •We identified various subsets of TFs that responded differentially through time and in an oxygen concentration-dependent manner. The regulatory potential of selected TFs and their predicted DNA binding elements was validated. Although the expression of pri-miRNAs was differentially regulated under hypoxia, only one corresponding mature miRNA changed accordingly. Putative target transcripts of the miRNAs were not significantly affected.
- •Our results show that the regulation of hypoxia-induced genes is controlled via simultaneous interaction of various combinations of TFs. Under anoxic conditions, an additional set of TFs is induced. Regulation of gene expression via miRNAs appears to play a minor role during hypoxia.