Fig. S1 Western blot detection of the brome mosaic virus (BMV) coat protein in systemically infected maize leaves.

Fig. S2 Virus-induced gene silencing (VIGS) of maize endochitinase B (ecb) and Bowmann–Birk type trypsin inhibitor (bti) genes.

Fig. S3 PCR on brome mosaic virus (BMV) RNA3 with insertion of a bax inhibitor 1 (bi-1) fragment to demonstrate insert stability of silencing constructs in systemically infected maize leaves.

Table S1 List of PCR primers used in this study

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