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Fig. S1 Effect of the nitric oxide (NO) scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) on cold-regulated gene expression in chilled Arabidopsis thaliana Columbia (Col-0) leaves.

Fig. S2 Thin-layer chromatography (TLC) patterns of labeled phospholipids extracted from cell cultures treated with 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO).

Fig. S3 Thin-layer chromatography (TLC) patterns of labeled phosphosphingolipids extracted from cell cultures treated with 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) or nitroso-gluthatione (GSNO).

Fig. S4 Effect of glutathione (GSH) treament on cold-induced phytosphingosine phosphate (PHS-P) and ceramide phosphate (Cer-P) synthesis.

Fig. S5 Thin-layer chromatography (TLC) patterns of labeled phosphosphingolipids of wild-type, nia1nia2 and H7 plantlets subjected or not subjected to chilling.

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