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Jasmonate biosynthesis in legume and actinorhizal nodules

Authors

  • Anna Zdyb,

    1. Georg-August-University Göttingen, Albrecht-von-Haller Institute for Plant Sciences, Department of Plant Biochemistry, 37077 Göttingen, Germany
    2. Stockholm University, Department of Botany, 106 91 Stockholm, Sweden
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    • These authors contributed equally to this work.

  • Kirill Demchenko,

    1. Georg-August-University Göttingen, Albrecht-von-Haller Institute for Plant Sciences, Department of Plant Biochemistry, 37077 Göttingen, Germany
    2. Komarov Botanical Institute, Russian Academy of Sciences, 197376 St. Petersburg, Russia
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    • These authors contributed equally to this work.

  • Jan Heumann,

    1. Leibniz Institute of Plant Biochemistry, Department of Secondary Metabolism, 06120 Halle (Saale), Germany
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    • These authors contributed equally to this work.

  • Cornelia Mrosk,

    1. Leibniz Institute of Plant Biochemistry, Department of Secondary Metabolism, 06120 Halle (Saale), Germany
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  • Peter Grzeganek,

    1. Georg-August-University Göttingen, Albrecht-von-Haller Institute for Plant Sciences, Department of Plant Biochemistry, 37077 Göttingen, Germany
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  • Cornelia Göbel,

    1. Georg-August-University Göttingen, Albrecht-von-Haller Institute for Plant Sciences, Department of Plant Biochemistry, 37077 Göttingen, Germany
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  • Ivo Feussner,

    1. Georg-August-University Göttingen, Albrecht-von-Haller Institute for Plant Sciences, Department of Plant Biochemistry, 37077 Göttingen, Germany
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  • Katharina Pawlowski,

    1. Georg-August-University Göttingen, Albrecht-von-Haller Institute for Plant Sciences, Department of Plant Biochemistry, 37077 Göttingen, Germany
    2. Stockholm University, Department of Botany, 106 91 Stockholm, Sweden
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  • Bettina Hause

    1. Leibniz Institute of Plant Biochemistry, Department of Secondary Metabolism, 06120 Halle (Saale), Germany
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Author for correspondence:
Katharina Pawlowski
Tel: +46 8 16 3772
Email: pawlowski@botan.su.se

Summary

  • Jasmonic acid (JA) is a plant signalling compound that has been implicated in the regulation of mutualistic symbioses. In order to understand the spatial distribution of JA biosynthetic capacity in nodules of two actinorhizal species, Casaurina glauca and Datisca glomerata, and one legume, Medicago truncatula, we determined the localization of allene oxide cyclase (AOC) which catalyses a committed step in JA biosynthesis. In all nodule types analysed, AOC was detected exclusively in uninfected cells.
  • The levels of JA were compared in the roots and nodules of the three plant species. The nodules and noninoculated roots of the two actinorhizal species, and the root systems of M. truncatula, noninoculated or nodulated with wild-type Sinorhizobium meliloti or with mutants unable to fix nitrogen, did not show significant differences in JA levels. However, JA levels in all plant organs examined increased significantly on mechanical disturbance.
  • To study whether JA played a regulatory role in the nodules of M. truncatula, composite plants containing roots expressing an MtAOC1-sense or MtAOC1-RNAi construct were inoculated with S. meliloti. Neither an increase nor reduction in AOC levels resulted in altered nodule formation.
  • These data suggest that jasmonates are not involved in the development and function of root nodules.

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