Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa

Authors

  • Liqiang Jia,

    1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China
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  • Zhongchang Wu,

    1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China
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  • Xi Hao,

    1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China
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  • Chris Carrie,

    1. ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, 6009 WA, Australia
    2. Joint Laboratory in Genomics and Nutriomics, College of Life Science, Zhejiang University, Hangzhou 310058, China
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  • Libin Zheng,

    1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China
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  • James Whelan,

    1. ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, 6009 WA, Australia
    2. Joint Laboratory in Genomics and Nutriomics, College of Life Science, Zhejiang University, Hangzhou 310058, China
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  • Yunrong Wu,

    1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China
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  • Shoufeng Wang,

    1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China
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  • Ping Wu,

    1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China
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  • Chuanzao Mao

    1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China
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Authors for correspondence:
Chuanzao Mao
Tel: +86 571 88206108
Email: mcz@zju.edu.cn

Ping Wu
Tel: +86 571 88206617
Email: clspwu@zju.edu.cn

Summary

  • A rice mutant, Oryza sativa short postembryonic roots 1 (Osspr1), has been characterized. It has short postembryonic roots, including adventitious and lateral roots, and a lower iron content in its leaves.
  • OsSPR1 was identified by map-based cloning. It encodes a novel mitochondrial protein with the Armadillo-like repeat domain.
  • Osspr1 mutants exhibited decreased root cell elongation. The iron content of the mutant shoots was significantly altered compared with that of wild-type shoots. A similar pattern of alteration of manganese and zinc concentrations in shoots was also observed. Complementation of the mutant confirmed that OsSPR1 is involved in post-embryonic root elongation and iron homeostasis in rice. OsSPR1 was found to be ubiquitously expressed in various tissues throughout the plant. The transcript abundance of various genes involved in iron uptake and signaling via both strategies I and II was similar in roots of wild-type and mutant plants, but was higher in the leaves of mutant plants.
  • Thus, a novel mitochondrial protein that is involved in root elongation and plays a role in metal ion homeostasis has been identified.

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