These authors contributed equally to this work as first authors.
Mitogen-activated protein kinase 4 is involved in the regulation of mitotic and cytokinetic microtubule transitions in Arabidopsis thaliana
Article first published online: 14 DEC 2010
© 2010 The Authors. New Phytologist © 2010 New Phytologist Trust
Special Issue: Featured papers on ‘Carbon cycling in tropical ecosystems’
Volume 189, Issue 4, pages 1069–1083, March 2011
How to Cite
Beck, M., Komis, G., Ziemann, A., Menzel, D. and Šamaj, J. (2011), Mitogen-activated protein kinase 4 is involved in the regulation of mitotic and cytokinetic microtubule transitions in Arabidopsis thaliana. New Phytologist, 189: 1069–1083. doi: 10.1111/j.1469-8137.2010.03565.x
- Issue published online: 3 FEB 2011
- Article first published online: 14 DEC 2010
- Received: 28 May 2010, Accepted: 22 October 2010
- Arabidopsis thaliana;
- genetic knock-out;
- microtubule-associated protein;
- mitogen-activated protein kinase;
- pharmacological inhibition;
- •A mitogen-activated protein kinase kinase kinase (MAPKKK) double mutant, Arabidopsis homologue of nucleus and phragmoplast associated kinase (anp) anp2anp3, and the mitogen-activated protein kinase (MAPK) 4 mutant mpk4 of Arabidopsis thaliana show prominent cytokinetic defects. This prompted the analysis of mitotic and cytokinetic progression as a function of MAPK signalling. Mutants were compared with wild types untreated or treated with the specific MAPKK inhibitor PD98059.
- •This study included phenotype analysis, expression analysis of the MPK4 promoter, immunofluorescent localization of MPK4, tubulin and MAP65-1, and time-lapse microscopic visualization of the mitotic microtubule (MT) transitions in control, mutant and inhibitor-treated cells.
- •Mutant and inhibitor-treated cells showed defects in mitosis and cytokinesis, including aberrant spindle and phragmoplast formation and drastically delayed or abortive mitosis and cytokinesis. As a result, bi- and multinucleate cells were formed, ultimately disturbing the vegetative tissue patterning. MPK4 was localized to all stages of the expanding phragmoplast, in a pattern similar to that of its putative substrate MAP65-1.
- •In this study, MPK4 is shown to be involved in the regulation of mitosis/cytokinesis through modulation of the cell division plane and cytokinetic progression.