These authors contributed equally to this work.
Arabidopsis VILLIN4 is involved in root hair growth through regulating actin organization in a Ca2+-dependent manner
Article first published online: 28 JAN 2011
© 2011 The Authors. New Phytologist © 2011 New Phytologist Trust
Volume 190, Issue 3, pages 667–682, May 2011
How to Cite
Zhang, Y., Xiao, Y., Du, F., Cao, L., Dong, H. and Ren, H. (2011), Arabidopsis VILLIN4 is involved in root hair growth through regulating actin organization in a Ca2+-dependent manner. New Phytologist, 190: 667–682. doi: 10.1111/j.1469-8137.2010.03632.x
- Issue published online: 18 APR 2011
- Article first published online: 28 JAN 2011
- Received: 18 October 2010, Accepted: 12 December 2010
- actin-binding protein;
- actin bundle;
- actin cytoskeleton;
- cytoplasmic streaming;
- •Villin is one of the major actin filament bundling proteins in plants. The function of Arabidopsis VILLINs (AtVLNs) is still poorly understood in living cells. In this report, the biochemical activity and cellular function of AtVLN4 were examined.
- •The biochemical property of AtVLN4 was characterized by co-sedimentation assays, fluorescence microscopy and spectroscopy of pyrene fluorescence. The in vivo function of AtVLN4 was analysed by ectopically expressing it in tobacco pollen and examining the phenotypes of its T-DNA insertional plants.
- •Recombinant AtVLN4 protein exhibited multiple activities on actin, including actin filament bundling, calcium (Ca2+)-dependent filament severing and barbed end capping. Expression of AtVLN4 in tobacco pollen induced the formation of supernumerary actin cables and reduced pollen tube growth. Loss of function of AtVLN4 resulted in slowing of root hair growth, alteration in cytoplasmic streaming routes and rate, and reduction of both axial and apical actin bundles.
- •Our results demonstrated that AtVLN4 is involved in root hair growth through regulating actin organization in a Ca2+-dependent manner.