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Fig. S1 Schematic representation of the exon structure of predicted rice oxidosqualene cyclase genes and pseudogenes.

Fig. S2 Schematic representation of the exon structure of predicted rice cytochrome P450 51 genes and pseudogenes.

Fig. S3 Phylogenetic tree estimates for the rice oxidosqualene genes obtained from the RAxML coding sequence analysis using the GTR+Γ+I substitution model (a), the MrBayes amino acid analysis using the WAG+Γ+I substitution model (b) and the MrBayes coding sequence analysis using the GTR+Γ+I substitution model (c).

Fig. S4 Alignment of the 14 oat and rice oxidosqualene cyclase protein sequences.

Fig. S5 Phylogenetic tree estimates for the rice cytochrome P450 51 genes obtained from the RAxML cds analysis using the GTR+Γ+I substitution model (a), the MrBayes amino acid analysis using the WAG+Γ+I substitution model (b) and the MrBayes coding sequence analysis using the GTR+Γ+I substitution model (c).

Fig. S6 Alignment of the 14 oat and rice cytochrome P450 51 protein sequences (with the first 51 positions unaligned because of the high level of amino acid variation).

Table S1 Database accession numbers for the oxidosqualene cyclase (OSC) and cytochrome P450 51 (CYP51) sequences used in this analysis

Table S2 PCR primers used for gene expression analysis

Table S3 Lengths of exons and introns within the rice oxidosqualene cyclase genes

Table S4 Predicted amino acid and coding sequences for the rice oxidosqualene cyclase genes, together with comments on the prediction process

Table S5 Lengths of exons and introns within the rice cytochrome P450 51 genes

Table S6 Predicted amino acid and coding sequences for the rice cytochrome P450 51 genes, together with comments on the prediction process

Table S7 Results from the PAML branch-site tests on the rice oxidosqualene cyclase genes

Table S8 Results from the PAML GC composition analysis on the rice oxidosqualene cyclase genes

Table S9 Results from the PAML pairwise maximum likelihood estimates of synonymous and nonsynonymous substitution rates for the rice oxidosqualene cyclase genes

Table S10 Results from the PAML branch-site tests on the rice cytochrome P450 51 genes

Table S11 Results from the PAML GC composition analysis on the rice cytochrome P450 51 genes

Table S12 Results from the PAML pairwise maximum likelihood estimates of synonymous and nonsynonymous substitution rates for the rice cytochrome P450 51 genes

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