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Fig. S1 Distribution of subcellular localization of Tsip1::GFP in Nicotiana benthamiana.

Fig. S2 Visualizaton of CMV 1a and 2a interaction by BiFC assay.

Fig. S3 Interaction strength comparison of Tsip1 with CMV 1a and CMV 2a by BiFC.

Fig. S4 RT-PCR analysis of Tsip1 expression in 35S::Tsip1 and Tsip1-RNAi transgenic tobacco lines upon SA treatment.

Fig. S5 CMV 1a and 2a proteins normally localize to the vacuolar membrane in the Tsip1 overexpression plants.

Fig. S6 3, 3′-diaminobenzidine (DAB) staining analysis for detecting hydrogen peroxide accumulation between Tsip1 transgenic tobacco and wild type plants.

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