A zinc finger protein Tsip1 controls Cucumber mosaic virus infection by interacting with the replication complex on vacuolar membranes of the tobacco plant
Article first published online: 8 APR 2011
© 2011 The Authors. New Phytologist © 2011 New Phytologist Trust
Volume 191, Issue 3, pages 746–762, August 2011
How to Cite
Huh, S. U., Kim, M. J., Ham, B.-K. and Paek, K.-H. (2011), A zinc finger protein Tsip1 controls Cucumber mosaic virus infection by interacting with the replication complex on vacuolar membranes of the tobacco plant. New Phytologist, 191: 746–762. doi: 10.1111/j.1469-8137.2011.03717.x
- Issue published online: 20 JUL 2011
- Article first published online: 8 APR 2011
- Received: 4 January 2011, Accepted: 4 March 2011
Fig. S1 Distribution of subcellular localization of Tsip1::GFP in Nicotiana benthamiana.
Fig. S2 Visualizaton of CMV 1a and 2a interaction by BiFC assay.
Fig. S3 Interaction strength comparison of Tsip1 with CMV 1a and CMV 2a by BiFC.
Fig. S4 RT-PCR analysis of Tsip1 expression in 35S::Tsip1 and Tsip1-RNAi transgenic tobacco lines upon SA treatment.
Fig. S5 CMV 1a and 2a proteins normally localize to the vacuolar membrane in the Tsip1 overexpression plants.
Fig. S6 3, 3′-diaminobenzidine (DAB) staining analysis for detecting hydrogen peroxide accumulation between Tsip1 transgenic tobacco and wild type plants.
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