Fig. S1 Homoeologous markers were used to assess the number of copies of chromosomes A1 and C1 in resynthesized Brassica napus.

Fig. S2 Distribution of estimated chromosome number in individuals from the cross between F1-EMZ and cv Darmor using flow cytometer.

Fig. S3 Comparison between male (Bn × CD.S0) and female (CD.S0 × Bn) meioses from an identical resynthesized Brassica napus plant.

Fig. S4 The most frequent rearrangement types following a first round of meiosis in different resynthesized Brassica napus.

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