The sucrose-regulated Arabidopsis transcription factor bZIP11 reprograms metabolism and regulates trehalose metabolism

Authors

  • Jingkun Ma,

    1. Centre for Signal Transduction and Metabolomics, Institute of Botany, The Chinese Academy of Sciences, Naxincun 20, Beijing 100093, China
    2. Department of Molecular Plant Physiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands
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  • Micha Hanssen,

    1. Department of Molecular Plant Physiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands
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  • Krister Lundgren,

    1. Umeå Plant Science Center, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-901 87 Umeå, Sweden
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  • Lázaro Hernández,

    1. Department of Molecular Plant Physiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands
    2. Centre for Genetic Engineering and Biotechnology (CIGB), PO Box 6162, CP 10600, Havana, Cuba
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  • Thierry Delatte,

    1. Department of Molecular Plant Physiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands
    2. Department of Biomedical Analysis, Utrecht University, 3584 CA Utrecht, the Netherlands
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  • Andrea Ehlert,

    1. Julius-Maximilians-Universität Würzburg, Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl für Pharmazeutische Biologie, Molekularbiologie und Biotechnologie der Pflanze, Julius-von-Sachs Platz 2, 97082 Würzburg, Germany
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  • Chun-Ming Liu,

    1. Centre for Signal Transduction and Metabolomics, Institute of Botany, The Chinese Academy of Sciences, Naxincun 20, Beijing 100093, China
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  • Henriette Schluepmann,

    1. Department of Molecular Plant Physiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands
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  • Wolfgang Dröge-Laser,

    1. Julius-Maximilians-Universität Würzburg, Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl für Pharmazeutische Biologie, Molekularbiologie und Biotechnologie der Pflanze, Julius-von-Sachs Platz 2, 97082 Würzburg, Germany
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  • Thomas Moritz,

    1. Umeå Plant Science Center, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-901 87 Umeå, Sweden
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  • Sjef Smeekens,

    1. Department of Molecular Plant Physiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands
    2. Centre for BioSystems Genomics, POB 98, 6700 AB Wageningen, the Netherlands
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  • Johannes Hanson

    1. Department of Molecular Plant Physiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands
    2. Centre for BioSystems Genomics, POB 98, 6700 AB Wageningen, the Netherlands
    3. Umeå Plant Science Center, Department of Physiological Botany, Umeå University, SE-901 87 Umeå, Sweden
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Author for correspondence:
Johannes Hanson
Tel: +31 30 253 3132
Email: s.j.hanson@uu.nl

Summary

  • The Arabidopsis basic region-leucine zipper transcription factor 11 (bZIP11) is known to be repressed by sucrose through a translational inhibition mechanism that requires the conserved sucrose control peptide encoded by the mRNA leader. The function of bZIP11 has been investigated in over-expression studies, and bZIP11 has been found to inhibit plant growth. The addition of sugar does not rescue the growth inhibition phenotype. Here, the function of the bZIP11 transcription factor was investigated.
  • The mechanism by which bZIP11 regulates growth was studied using large-scale and dedicated metabolic analysis, biochemical assays and molecular studies.
  • bZIP11 induction results in a reprogramming of metabolism and activation of genes involved in the metabolism of trehalose and other minor carbohydrates such as myo-inositol and raffinose. bZIP11 induction leads to reduced contents of the prominent growth regulatory molecule trehalose 6-phosphate (T6P).
  • The metabolic changes detected mimic in part those observed in carbon-starved plants. It is proposed that bZIP11 is a powerful regulator of carbohydrate metabolism that functions in a growth regulatory network that includes T6P and the sucrose non-fermenting-1 related protein kinase 1 (SnRK1).

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