Fig. S1 Auto-oxidation of (9Z)-traumatin in vitro.

Fig. S2 Determination of the linearity and limit of detection of C12 metabolites.

Fig. S3 Accumulation of C12 derivatives in leaves of wild-type (WT), ir-lox2 and ir-hpl plants after wounding and fatty acid–amino acid conjugate (FAC) elicitation.

Fig. S4 Calibration curves for C12 standards.

Fig. S5 Quantification of Nicotiana attenuata hydroperoxide lyase (NaHPL) mRNA levels in wild-type (WT) and ir-hpl plants.

Fig. S6 Southern blot analysis of ir-hpl plants.

Table S1 Parameters used for detection of C12 molecules by liquid chromatography–mass spectrometry/mass spectrometry (LC-MS/MS)

Table S2 Parameters used for quantification of C12 molecules by liquid chromatography–mass spectrometry/mass spectrometry (LC-MS/MS)

Table S3 Complete list of genes changing expression in wounded leaves of ir-lox2/ir-lox3 plants after treatment with 9Z-traumatin : 9-OH-traumatin (1 : 1)

Table  S4 Analysis of C6 volatiles emitted by wild-type (WT) and ir-hpl plants

Methods S1 Characterization of ir-hpl plants.

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