Type-2 histone deacetylases as new regulators of elicitor-induced cell death in plants

Authors

  • Stéphane Bourque,

    1. UMR INRA 1088/CNRS 5184/Université de Bourgogne Plante–Microbe–Environnement, 17 Rue Sully, BP 86510, 21065 Dijon cedex, France
    2. GDR CNRS N°2688 ‘Calcium et régulation de l’expression des gènes en contexte normal et pathologique’, 31000 Toulouse, France
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    • These authors contributed equally to this work.

  • Agnès Dutartre,

    1. UMR INRA 1088/CNRS 5184/Université de Bourgogne Plante–Microbe–Environnement, 17 Rue Sully, BP 86510, 21065 Dijon cedex, France
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    • These authors contributed equally to this work.

  • Valentin Hammoudi,

    1. UMR INRA 1088/CNRS 5184/Université de Bourgogne Plante–Microbe–Environnement, 17 Rue Sully, BP 86510, 21065 Dijon cedex, France
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  • Sabrina Blanc,

    1. UMR INRA 1088/CNRS 5184/Université de Bourgogne Plante–Microbe–Environnement, 17 Rue Sully, BP 86510, 21065 Dijon cedex, France
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  • Jennifer Dahan,

    1. UMR INRA 1088/CNRS 5184/Université de Bourgogne Plante–Microbe–Environnement, 17 Rue Sully, BP 86510, 21065 Dijon cedex, France
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  • Sylvain Jeandroz,

    1. UPSP PROXISS, AgroSup Dijon, 26 Boulevard du Dr Petitjean, BP 87999, 21079 Dijon cedex, France
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  • Carole Pichereaux,

    1. Plateforme Protéomique Génopole Toulouse Midi-Pyrénées, Institut de Pharmacologie et de Biologie Structurale (IPBS), CNRS, 205 route de Narbonne, F-31077 Toulouse, France and Université Paul Sabatier, Université de Toulouse, F-31077 Toulouse, France
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  • Michel Rossignol,

    1. Plateforme Protéomique Génopole Toulouse Midi-Pyrénées, Institut de Pharmacologie et de Biologie Structurale (IPBS), CNRS, 205 route de Narbonne, F-31077 Toulouse, France and Université Paul Sabatier, Université de Toulouse, F-31077 Toulouse, France
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  • David Wendehenne

    1. UMR INRA 1088/CNRS 5184/Université de Bourgogne Plante–Microbe–Environnement, 17 Rue Sully, BP 86510, 21065 Dijon cedex, France
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  • Protein and nucleic acid sequences: NtHD2 sequences were deposited at GenBank. NtHD2a, FJ903174; NtHD2b, FJ903175.

Author for correspondence:
Stéphane Bourque
Tel: +33 3 80 69 34 76
Email: bourque@dijon.inra.fr

Summary

  • Plant resistance to pathogen attack is often associated with a localized programmed cell death called hypersensitive response (HR). How this cell death is controlled remains largely unknown.
  • Upon treatment with cryptogein, an elicitor of tobacco defence and cell death, we identified NtHD2a and NtHD2b, two redundant isoforms of type-2 nuclear histone deacetylases (HDACs). These HDACs are phosphorylated after a few minutes’ treatment, and their rate of mRNAs are rapidly and strongly reduced, leading to a 40-fold decrease after10 h of treatment.
  • By using HDAC inhibitors, RNAi- and overexpression-based approaches, we showed that HDACs, and especially NtHD2a/b, act as inhibitors of cryptogein-induced cell death. Moreover, in NtHD2a/b-silenced plants, infiltration with cryptogein led to HR-like symptoms in distal leaves.
  • Taken together, these results show for the first time that type-2 HDACs, which are specific to plants, act as negative regulators of elicitor-induced cell death in tobacco (Nicotiana tabacum), suggesting that the HR is controlled by post-translational modifications including (de)acetylation of nuclear proteins.

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