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Fig. S1 Alignment of all NUNSSP-like clones from Nicotiana undulata, N. wigandioides and N. arentsi; alignment of NUNSSP-like clones from N. arentsii; alignment of NUNSSP clones from N. undulata; alignment of NWISSP clones from N. wigandioides.

Fig. S2 Detection of satellites in Nicotiana arentsii (A) and N. wigandioides (W).

Fig. S3 Phylogenic relationships between satellite monomers derived from the individual species.

Fig. S4 Gel retardation analysis example.

Table S1 List of best-fitting nucleotide substitution models

Table S2 Nucleotide substitution models used for the phylogenetic tree calculations

Table S3 Test of differences in sequence distances between individual groups of pairwise comparisons

Table S4 Mobility of individual NUNSSP-like monomers

Table S5 (a) Descriptive statistics of the mobility of selected groups of cloned monomers; and (b) test of differences between the mobility of individual groups of cloned monomers

Table S6 Descriptive statistics of the densitometric profiles of hybridization signals along each band of permutated genomic NUNSSP-like monomers

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