Tissue-specific expression of Populus C19 GA 2-oxidases differentially regulate above- and below-ground biomass growth through control of bioactive GA concentrations
Version of Record online: 5 AUG 2011
© 2011 The Authors. New Phytologist © 2011 New Phytologist Trust
Volume 192, Issue 3, pages 626–639, November 2011
How to Cite
Gou, J., Ma, C., Kadmiel, M., Gai, Y., Strauss, S., Jiang, X. and Busov, V. (2011), Tissue-specific expression of Populus C19 GA 2-oxidases differentially regulate above- and below-ground biomass growth through control of bioactive GA concentrations. New Phytologist, 192: 626–639. doi: 10.1111/j.1469-8137.2011.03837.x
- Issue online: 19 OCT 2011
- Version of Record online: 5 AUG 2011
- Received: 21 April 2011, Accepted: 14 June 2011
Fig. S1 Sequence fragments used in generation of RNAi 1–6, RNAi 2–7, RNAi 3 and RNAi 4–5.
Fig. S2 Sequence alignment and position of the RNAi-targeted sequences.
Fig. S3 Selection of optimal PCR cycles for the RT-PCR quantification of gene expression.
Fig. S4 Phylogenetic analysis of the GA2ox families in Populus and Arabidopsis.
Fig. S5 Associations of gene suppression and phenotypes in RNAi transgenic plants.
Fig. S6 Associations of phenotypes and expression of untargeted GA2ox genes in the RNAi 2–7 transgenics.
Fig. S7 Associations of phenotypes and expression of untargeted GA2ox genes in the RNAi 4–5 transgenics.
Table S1 Primers used to amplify the 4 fragments for generation of the RNAi constructs
Table S2 Primers used for RT-PCR expression analyses
Table S3 Gene models used in the phylogenetic analysis
Table S4 Protein sequences used in the phylogenetic analyses
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|NPH_3837_sm_TableS1-S4.xls||40K||Supporting info item|