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Fig. S1 Structures of the pyrrolizidine alkaloids (PAs) found in shoots and roots of Jacobaea aquatica, Jacobaea vulgaris, and F1 and F2 hybrids.

Fig. S2 Putative biosynthetic pathways for diversification of pyrrolizidine alkaloids (PAs) in the Jacobaea section.

Fig. S3 Frequency distribution of genotypic mean concentrations of pyrrolizidine alkaloids (PAs) from four structural groups in the shoots and roots of 102 F2 hybrid genotypes between Jacobaea aquatica and Jacobaea vulgaris.

Fig. S4 Pyrrolizidine alkaloid (PA) composition in the shoots and roots plotted by two-dimension nonparametric multidimensional scaling (NMDS) and the loadings.

Table S1 Pyrrolizidine alkaloid (PA) concentrations in the shoots of Jacobaea aquatic (one genotype), Jacobaea vulgaris (one genotype), F1 hybrids (two genotypes) and F2 hybrids (102 genotypes)

Table S2 Pyrrolizidine alkaloid (PA) concentrations in the roots of Jacobaea aquatic (one genotype), Jacobaea vulgaris (one genotype), F1 hybrids (two genotypes) and F2 hybrids (102 genotypes)

Table S3 Quantitative variation of pyrrolizidine alkaloids (PAs) in the shoots and roots of two F1 and 102 F2 hybrids relative to parental genotypes (one genotype each of Jacobaea aquatica and Jacobaea vulgaris)

Table S4 Coefficients (rs) of Spearman rank correlation between the individual pyrrolizidine alkaloid (PA) concentrations in the shoots and roots of Jacobaea aquatic (one genotype), Jacobaea vulgaris (one genotype), F1 hybrids (two genotypes) and F2 hybrids (102 genotypes)

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