Advertisement

Myo-inositol hexakisphosphate, isolated from female gametophyte tissue of loblolly pine, inhibits growth of early-stage somatic embryos

Authors

  • Di Wu,

    1. School of Chemistry and Biochemistry, Petit Institute for Bioengineering and Biosciences, School of Biology, and Institute of Paper Science and Technology, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
    Search for more papers by this author
  • M. Cameron Sullards,

    1. School of Chemistry and Biochemistry, Petit Institute for Bioengineering and Biosciences, School of Biology, and Institute of Paper Science and Technology, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
    Search for more papers by this author
  • Charlie D. Oldham,

    1. School of Chemistry and Biochemistry, Petit Institute for Bioengineering and Biosciences, School of Biology, and Institute of Paper Science and Technology, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
    Search for more papers by this author
  • Les Gelbaum,

    1. School of Chemistry and Biochemistry, Petit Institute for Bioengineering and Biosciences, School of Biology, and Institute of Paper Science and Technology, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
    Search for more papers by this author
  • Jacob Lucrezi,

    1. School of Chemistry and Biochemistry, Petit Institute for Bioengineering and Biosciences, School of Biology, and Institute of Paper Science and Technology, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
    Search for more papers by this author
  • Gerald S. Pullman,

    1. School of Chemistry and Biochemistry, Petit Institute for Bioengineering and Biosciences, School of Biology, and Institute of Paper Science and Technology, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
    Search for more papers by this author
  • Sheldon W. May

    1. School of Chemistry and Biochemistry, Petit Institute for Bioengineering and Biosciences, School of Biology, and Institute of Paper Science and Technology, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
    Search for more papers by this author

Author for correspondence:
Sheldon W. May
Tel: +1 404 894 4052
Email: sheldon.may@chemistry.gatech.edu

Summary

  • Myo-inositol hexakisphosphate (InsP6), abundant in animals and plants, is well known for its anticancer activity. However, many aspects of InsP6 function in plants remain undefined. We now report the first evidence that InsP6 can inhibit cellular proliferation in plants under growth conditions where phosphorus is not limited.
  • A highly anionic molecule inhibitory to early-stage somatic embryo growth of loblolly pine (LP) was purified chromatographically from late-stage LP female gametophytes (FGs), and then characterized structurally using mass spectrometry (MS) and nuclear magnetic resonance (NMR) analyses.
  • Exact mass and mass spectrometry-mass spectrometry (MS-MS) fragmentation identified the bioactive molecule as an inositol hexakisphosphate. It was then identified as the myo-isomer (i.e. InsP6) on the basis of 1H-, 31P- and 13C-NMR, 1H-1H correlation spectroscopy (COSY), 1H-31P heteronuclear single quantum correlation (HSQC) and 1H-13C HSQC. Topical application of InsP6 to early-stage somatic embryos indeed inhibits embryonic growth.
  • Recently evidence has begun to emerge that InsP6 may also play a regulatory role in plant cells. We anticipate that our findings will help to stimulate additional investigations aimed at elucidating the roles of inositol phosphates in cellular growth and development in plants.

Ancillary