These authors contributed equally to this work.
The transcriptome of the arbuscular mycorrhizal fungus Glomus intraradices (DAOM 197198) reveals functional tradeoffs in an obligate symbiont
Article first published online: 16 NOV 2011
© 2011 The Authors. New Phytologist © 2011 New Phytologist Trust
Volume 193, Issue 3, pages 755–769, February 2012
How to Cite
Tisserant, E., Kohler, A., Dozolme-Seddas, P., Balestrini, R., Benabdellah, K., Colard, A., Croll, D., Da Silva, C., Gomez, S. K., Koul, R., Ferrol, N., Fiorilli, V., Formey, D., Franken, Ph., Helber, N., Hijri, M., Lanfranco, L., Lindquist, E., Liu, Y., Malbreil, M., Morin, E., Poulain, J., Shapiro, H., van Tuinen, D., Waschke, A., Azcón-Aguilar, C., Bécard, G., Bonfante, P., Harrison, M. J., Küster, H., Lammers, P., Paszkowski, U., Requena, N., Rensing, S. A., Roux, C., Sanders, I. R., Shachar-Hill, Y., Tuskan, G., Young, J. P. W., Gianinazzi-Pearson, V. and Martin, F. (2012), The transcriptome of the arbuscular mycorrhizal fungus Glomus intraradices (DAOM 197198) reveals functional tradeoffs in an obligate symbiont. New Phytologist, 193: 755–769. doi: 10.1111/j.1469-8137.2011.03948.x
- Issue published online: 11 JAN 2012
- Article first published online: 16 NOV 2011
- Received: 31 August 2011, Accepted: 26 September 2011
- meiosis and recombination genes;
- small secreted proteins;
- transcript profiling
- •The arbuscular mycorrhizal symbiosis is arguably the most ecologically important eukaryotic symbiosis, yet it is poorly understood at the molecular level. To provide novel insights into the molecular basis of symbiosis-associated traits, we report the first genome-wide analysis of the transcriptome from Glomus intraradices DAOM 197198.
- •We generated a set of 25 906 nonredundant virtual transcripts (NRVTs) transcribed in germinated spores, extraradical mycelium and symbiotic roots using Sanger and 454 sequencing. NRVTs were used to construct an oligoarray for investigating gene expression.
- •We identified transcripts coding for the meiotic recombination machinery, as well as meiosis-specific proteins, suggesting that the lack of a known sexual cycle in G. intraradices is not a result of major deletions of genes essential for sexual reproduction and meiosis. Induced expression of genes encoding membrane transporters and small secreted proteins in intraradical mycelium, together with the lack of expression of hydrolytic enzymes acting on plant cell wall polysaccharides, are all features of G. intraradices that are shared with ectomycorrhizal symbionts and obligate biotrophic pathogens.
- •Our results illuminate the genetic basis of symbiosis-related traits of the most ancient lineage of plant biotrophs, advancing future research on these agriculturally and ecologically important symbionts.