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Fig. S1 Flow chart describing the in silico filtering process of Glomus intraradices expressed sequence tags (ESTs).

Fig. S2 GC% distribution of Glomus intraradices raw and filtered expressed sequence tag (EST) sequences for each cDNA library.

Fig. S3 Rarefaction curves depicting the effect of Glomus intraradices read number (a) on the number of assembled nonredundant virtual transcripts (NRVTs) and (b) on the number of detected proteins in the Swiss-Prot database and Rhizopus oryzae gene repertoire.

Fig. S4 Similarity of Glomus intraradices nonredundant virtual transcripts (NRVTs) to gene coding sequences from other selected fungi.

Table S1 The number of assembled nonredundant virtual transcripts (NRVTs) was affected by the parameters used for assembling the Glomus intraradices expressed sequence tags (ESTs) using the MIRA assembler

Table S2 Genes missing in the Glomus intraradices transcriptome, and gene repertoires of Blumeria graminis (Spanu et  al., 2010) and other obligate biotrophic pathogens (Kemen et  al., 2011)

Table S3 Most highly up-regulated Glomus intraradices transcripts in the extraradical mycelium (ERM) vs germinated spores identified using expression oligoarrays and ranked by decreasing fold changes

Table S4 All transcripts with functional annotations that were up-regulated by at least 10-fold in Glomus intraradices extraradical mycelium (ERM) compared with germinated spores based on oligoarray profiling (ratio ≥ 10; P-value < 0.05)

Table S5 Expression profiles, as obtained by semiquantitative RT-PCR, of genes showing up-regulation in Glomus intraradices intraradical mycelium (IRM) compared with extraradical mycelium (ERM) in oligoarray profiling

Table S6 Expression of nonredundant virtual transcripts (NRVTs) coding for carbohydrate-active enzymes (Cantarel et  al., 2009) identified in the Glomus intraradices transcriptome in germinated spores, extraradical mycelium (ERM) and intraradical mycelium (IRM)

Table S7 Expression of nonredundant virtual transcripts (NRVTs) coding for membrane transporters identified in the Glomus intraradices transcriptome in germinated spores, extraradical mycelium (ERM) and intraradical mycelium (IRM)

Table S8 Expression of nonredundant virtual transcripts (NRVTs) coding for transporters and enzymes involved in nitrogen metabolism in the Glomus intraradices transcriptome

Table S9 Expression of nonredundant virtual transcripts (NRVTs) coding for transporters and enzymes involved in phosphate metabolism in the Glomus intraradices transcriptome

Table S10 Expression of nonredundant virtual transcripts (NRVTs) coding for lipid metabolism enzymes in the Glomus intraradices transcriptome

Table S11 Expression levels and regulation ratio of transcripts related to meiosis in the Glomus intraradices transcriptome

Methods S1 Construction of the cDNA libraries.

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NPH_3948_sm_FigS1-S4.pdf4426KSupporting info item
NPH_3948_sm_MethodsS1.doc102KSupporting info item
NPH_3948_sm_TableS1-S11.doc823KSupporting info item