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Fig. S1 Immunoblot analysis of Pseudomonas syringae pv. tomato (Pto) T1 T3SE-HA proteins and avirulence D (AvrD)-HA expressed in PFO1-T3SS.

Fig. S2Pseudomonas syringae pv. tomato (Pto) T1 T3SEs, avrRpt2 and hopAS1, confer reduced virulence when delivered from Pto DC3000 in Arabidopsis accession Columbia (Col-0).

Fig. S3 HopAS1 confers reduced disease symptom development when delivered from Pseudomonas syringae pv. tomato (Pto) DC3000 in wild-type or mutant Arabidopsis accession Columbia (Col-0).

Fig. S4 Expression of SAX does not confer enhance growth of Pseudomonas syringae pv. tomato (Pto) T1 ∆hopAS1 or ∆hopAS1avrRpt2 in Arabidopsis.

Table S1 List of Pseudomonas syringae pv. tomato (Pto) T1 type III effectors analyzed in this study

Table S2 List of Arabidopsis accessions tested for hypersensitive response (HR) in responses to PFO1-T3SS carrying hopAS1PtoT1, avrRpm1, avrRpt2 or avrPphB

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