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Fig. S1 Soil moisture variation during the drought-tolerance experiment.

Fig. S2 Frequency distribution of relative DNA content (pg) obtained from the screening of 1274 individual plants from 637 Brachypodium distachyon accessions collected in 57 populations across the Iberian Peninsula.

Fig. S3 Simultaneous flow cytometry analysis of DAPI-stained nuclei isolated from diploid and tetraploid plants.

Table S1 Localities and geographical coordinates of the 57 Brachypodium distachyon populations across the Iberian Peninsula used in this study

Table S2 Ploidy level variation among 637 Brachypodium distachyon accessions collected from 57 populations in the Iberian Peninsula

Table S3 Location and geographical provenance of the 96 genotypes used in the assessment of the variation in water-use efficiency and flowering time among 24 natural Brachypodium distachyon populations across the Iberian Peninsula

Table S4 Spearman’s correlation coefficients among the geographical and climate parameters considered in this study

Table S5 Principal components analysis conducted on two climatic and three geographical parameters of 57 Brachypodium distachyon populations across the Iberian Peninsula

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