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Fig. S1 Predicted species range for four live oak species based on precipitation and temperature.

Fig. S2 Leaf decline in Fv/Fm after freezing (− 10°C) and stem freezing injury at five freezing temperatures for temperate and tropical growth treatment.

Fig. S3 Regressions for leaf and stem freezing tolerance and absolute growth rate (AGR) and relative growth rate (RGR) for both temperate and tropical treatments.

Fig. S4 Mean acorn mass for species and populations.

Table S1 Acorn collection site locations for all maternal families

Table S2 Restricted maximum likelihood (REML) variance component estimates for all response variables for the amount of variation explained by species and population for each growth temperature treatment

Methods S1 Leaf and stem freezing method details.

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