Phytosphingosine-phosphate is a signal for AtMPK6 activation and Arabidopsis response to chilling
Article first published online: 11 JAN 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Special Issue: Featured papers on ‘Bioenergy trees’
Volume 194, Issue 1, pages 181–191, April 2012
How to Cite
Dutilleul, C., Benhassaine-Kesri, G., Demandre, C., Rézé, N., Launay, A., Pelletier, S., Renou, J.-P., Zachowski, A., Baudouin, E. and Guillas, I. (2012), Phytosphingosine-phosphate is a signal for AtMPK6 activation and Arabidopsis response to chilling. New Phytologist, 194: 181–191. doi: 10.1111/j.1469-8137.2011.04017.x
- Issue published online: 24 FEB 2012
- Article first published online: 11 JAN 2012
- Received: 13 October 2011, Accepted: 18 November 2011
Fig. S1 Diagrams showing the position of the T-DNA insertion in the different Arabidopsis thaliana long-chain base (LCB) kinase mutant lines.
Fig. S2 Analysis of free (phospho)-long-chain base (LCB-(P)) profiles of cell cultures treated with 4-hydroxy-sphinganine-phosphate (PHS-P) and/or phytosphingosine (PHS).
Fig. S3 Mitogen-activated protein kinase (MAPK) activities and cold-responsive gene expression in wild-type (WT) and sphk2 mutants exposed to low temperatures.
Fig. S4 Regulation of NITRATE-INDUCED 3 (NOI3) gene expression by phytosphingosine/4-hydroxy-sphinganine-phosphate (PHS/PHS-P).
Table S1 Oligonucleotide primer sequences
Table S2 Relative abundance of radiolabelled 4-hydroxy-sphinganine-phosphate (PHS-P) in total radiolabelled phosphosphingolipids extracted from unstressed and cold-stressed (4°C) plantlets
Table S3 Relative abundance of radiolabelled 4-hydroxy-sphinganine-phosphate (PHS-P) in total radiolabelled phosphosphingolipids extracted from unstressed and cold-stressed (12°C) plantlets after 30 min of labelling
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