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Fig. S1 Diagrams showing the position of the T-DNA insertion in the different Arabidopsis thaliana long-chain base (LCB) kinase mutant lines.

Fig. S2 Analysis of free (phospho)-long-chain base (LCB-(P)) profiles of cell cultures treated with 4-hydroxy-sphinganine-phosphate (PHS-P) and/or phytosphingosine (PHS).

Fig. S3 Mitogen-activated protein kinase (MAPK) activities and cold-responsive gene expression in wild-type (WT) and sphk2 mutants exposed to low temperatures.

Fig. S4 Regulation of NITRATE-INDUCED 3 (NOI3) gene expression by phytosphingosine/4-hydroxy-sphinganine-phosphate (PHS/PHS-P).

Table S1 Oligonucleotide primer sequences

Table S2 Relative abundance of radiolabelled 4-hydroxy-sphinganine-phosphate (PHS-P) in total radiolabelled phosphosphingolipids extracted from unstressed and cold-stressed (4°C) plantlets

Table S3 Relative abundance of radiolabelled 4-hydroxy-sphinganine-phosphate (PHS-P) in total radiolabelled phosphosphingolipids extracted from unstressed and cold-stressed (12°C) plantlets after 30 min of labelling

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NPH_4017_sm_FigS1.tif3175KSupporting info item
NPH_4017_sm_FigS2.tif3175KSupporting info item
NPH_4017_sm_FigS3.tif1521KSupporting info item
NPH_4017_sm_FigS4.tif2931KSupporting info item
NPH_4017_sm_TableS1.doc26KSupporting info item
NPH_4017_sm_TableS2.doc33KSupporting info item
NPH_4017_sm_TableS3.doc29KSupporting info item