A dual-targeted purple acid phosphatase in Arabidopsis thaliana moderates carbon metabolism and its overexpression leads to faster plant growth and higher seed yield
Article first published online: 23 JAN 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Special Issue: Featured papers on ‘Bioenergy trees’
Volume 194, Issue 1, pages 206–219, April 2012
How to Cite
Sun, F., Suen, P. K., Zhang, Y., Liang, C., Carrie, C., Whelan, J., Ward, J. L., Hawkins, N. D., Jiang, L. and Lim, B. L. (2012), A dual-targeted purple acid phosphatase in Arabidopsis thaliana moderates carbon metabolism and its overexpression leads to faster plant growth and higher seed yield. New Phytologist, 194: 206–219. doi: 10.1111/j.1469-8137.2011.04026.x
- Issue published online: 24 FEB 2012
- Article first published online: 23 JAN 2012
- Received: 18 October 2011, Accepted: 28 November 2011
Fig. S1 Phylogenetic analysis of full length AtPAP2 protein sequence with related proteins in the other plant species.
Fig. S2 Amino acid sequence alignment of AtPAP2 with its homologous sequences from other plants.
Fig. S3 Verification of AtPAP2 T-DNA mutant line.
Fig. S4 Western blot analysis of AtPAP2 in Arabidopsis tissues.
Fig. S5 Expression of AtPAP2 under various treatments.
Fig. S6 Growth phenotypes of genetically modified Arabidopsis.
Fig. S7 The TMDCT region of AtPAP2 contains targeting signal to mitochondria.
Fig. S8 AtPAP2-GFP constructs did not co-localize with ER or Golgi markers.
Fig. S9 AtPAP2-GFP constructs did not co-localize with peroxisome marker.
Fig. S10 Analysis of SnRK1 proteins.
Fig. S11 Principal component analysis of 1H-NMR data and GC-MS data.
Table S1 Signature motifs of AtPAP2-like proteins
Table S2 Relative metabolite content of shoots of 20-d-old At-PAP2 OE lines
Table S3 Primers used in this study
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