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Fig. S1 Confirmation of expression of Arabidopsis histidine kinase 5 (AHK5) in genetically complemented lines by semiquantitative RT-PCR.

Fig. S2 Seedling survival assay in response to 200mM NaCl.

Fig. S3 Root growth of wild-type and Arabidopsis histidine kinase 5-1 mutant (ahk5-1) seedlings in response to sorbitol and KCl.

Fig. S4 Symptom development in wild-type Columbia (Col-0) and Arabidopsis histidine kinase 5-1 mutant (ahk5-1) leaves surface-inoculated with 4 × 108 cfu ml−1Pseudomonas syringae pv. tomato DC3000 (PstDC3000) 7 d post-inoculation.

Fig. S5 3,3-Diaminobenzidine (DAB) staining of fungal hyphae growing on wild-type and Arabidopsis histidine kinase 5-1 (ahk5-1) mutant leaves at 12 h post-inoculation.

Fig. S6 Trypan blue staining of fungal hyphae growing on wild-type and Arabidopsis histidine kinase 5-1 (ahk5-1) mutant leaves at 8 h post-inoculation.

Fig. S7 Lesion size in wild-type Columbia (Col-0) leaves 3 d post-inoculation with pre-(vacuum) infiltration of diphenyleneiodinium chloride (DPI; 1 μM) or 2-(4-carboxyphenyl)-4, 4, 4, 5-tetramethylimidazoline-oxyl-3-oxide (cPTIO; 50 μM) 2 h before inoculation of leaves with 2 × 105 spores ml−1Botrytis cinerea in 1/8th potato dextrose broth.

Fig. S8 Dose-dependent response of seedling root growth to exogenous ABA.

Fig. S9 Unique metabolite detected in leaf tissue of the Arabidopsis histidine kinase 5-1 (ahk5-1) mutant mock-inoculated with 10mM MgCl2 (control) or surface-inoculated with 4 × 108 cfu ml−1Pseudomonas syringae pv. tomato DC3000 (PstDC3000) by LC-MS/MS.

Fig. S10 Hyphal length of Botrytis cinerea spores germinated in 1/8th potato dextrose broth with or without the nitric oxide (NO) scavenger 2-(4-carboxyphenyl)-4, 4, 4, 5-tetramethylimidazoline-oxyl-3-oxide (cPTIO) (50 μM) for various durations.

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