These authors contributed equally to this work.
Plant genes involved in harbouring symbiotic rhizobia or pathogenic nematodes
Article first published online: 23 FEB 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 194, Issue 2, pages 511–522, April 2012
How to Cite
Damiani, I., Baldacci-Cresp, F., Hopkins, J., Andrio, E., Balzergue, S., Lecomte, P., Puppo, A., Abad, P., Favery, B. and Hérouart, D. (2012), Plant genes involved in harbouring symbiotic rhizobia or pathogenic nematodes. New Phytologist, 194: 511–522. doi: 10.1111/j.1469-8137.2011.04046.x
- Issue published online: 19 MAR 2012
- Article first published online: 23 FEB 2012
- Received: 13 October 2011, Accepted: 18 December 2011
- giant cell;
- laser microdissection;
- Medicago truncatula;
- nitrogen-fixing symbiosis;
- root-knot nematode (RKN);
- •The establishment and development of plant–microorganism interactions involve impressive transcriptomic reprogramming of target plant genes. The symbiont (Sinorhizobium meliloti) and the root knot-nematode pathogen (Meloidogyne incognita) induce the formation of new root organs, the nodule and the gall, respectively.
- •Using laser-assisted microdissection, we specifically monitored, at the cell level, Medicago gene expression in nodule zone II cells, which are preparing to receive rhizobia, and in gall giant and surrounding cells, which play an essential role in nematode feeding and constitute the typical root swollen structure, respectively.
- •We revealed an important reprogramming of hormone pathways and C1 metabolism in both interactions, which may play key roles in nodule and gall neoformation, rhizobia endocytosis and nematode feeding. Common functions targeted by rhizobia and nematodes were mainly down-regulated, whereas the specificity of the interaction appeared to involve up-regulated genes.
- •Our transcriptomic results provide powerful datasets to unravel the mechanisms involved in the accommodation of rhizobia and root-knot nematodes. Moreover, they raise the question of host specificity and the evolution of plant infection mechanisms by a symbiont and a pathogen.