Plant genes involved in harbouring symbiotic rhizobia or pathogenic nematodes

Authors

  • Isabelle Damiani,

    1. INRA, UMR 1301, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
    2. CNRS, UMR 6243, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
    3. Université de Nice Sophia-Antipolis, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
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  • Fabien Baldacci-Cresp,

    1. INRA, UMR 1301, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
    2. CNRS, UMR 6243, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
    3. Université de Nice Sophia-Antipolis, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
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  • Julie Hopkins,

    1. INRA, UMR 1301, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
    2. CNRS, UMR 6243, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
    3. Université de Nice Sophia-Antipolis, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
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  • Emilie Andrio,

    1. INRA, UMR 1301, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
    2. CNRS, UMR 6243, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
    3. Université de Nice Sophia-Antipolis, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
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  • Sandrine Balzergue,

    1. URGV UMR INRA 1165 – CNRS 8114 – UEVE, 2 rue Gaston Crémieux, CP 5708, F-91057 Evry Cedex, France
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  • Philippe Lecomte,

    1. INRA, UMR 1301, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
    2. CNRS, UMR 6243, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
    3. Université de Nice Sophia-Antipolis, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
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  • Alain Puppo,

    1. INRA, UMR 1301, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
    2. CNRS, UMR 6243, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
    3. Université de Nice Sophia-Antipolis, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
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  • Pierre Abad,

    1. INRA, UMR 1301, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
    2. CNRS, UMR 6243, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
    3. Université de Nice Sophia-Antipolis, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
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  • Bruno Favery,

    1. INRA, UMR 1301, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
    2. CNRS, UMR 6243, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
    3. Université de Nice Sophia-Antipolis, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
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    • These authors contributed equally to this work.

  • Didier Hérouart

    1. INRA, UMR 1301, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
    2. CNRS, UMR 6243, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
    3. Université de Nice Sophia-Antipolis, Interactions Biotiques et Santé Végétale, 400 route des Chappes, F-06903 Sophia Antipolis, France
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    • These authors contributed equally to this work.


Authors for correspondence:
Didier Hérouart
Tel: +33 492 386 636
Email: herouart@unice.fr
Bruno Favery
Tel: +33 492 386 464
Email: favery@sophia.inra.fr

Summary

  • The establishment and development of plant–microorganism interactions involve impressive transcriptomic reprogramming of target plant genes. The symbiont (Sinorhizobium meliloti) and the root knot-nematode pathogen (Meloidogyne incognita) induce the formation of new root organs, the nodule and the gall, respectively.
  • Using laser-assisted microdissection, we specifically monitored, at the cell level, Medicago gene expression in nodule zone II cells, which are preparing to receive rhizobia, and in gall giant and surrounding cells, which play an essential role in nematode feeding and constitute the typical root swollen structure, respectively.
  • We revealed an important reprogramming of hormone pathways and C1 metabolism in both interactions, which may play key roles in nodule and gall neoformation, rhizobia endocytosis and nematode feeding. Common functions targeted by rhizobia and nematodes were mainly down-regulated, whereas the specificity of the interaction appeared to involve up-regulated genes.
  • Our transcriptomic results provide powerful datasets to unravel the mechanisms involved in the accommodation of rhizobia and root-knot nematodes. Moreover, they raise the question of host specificity and the evolution of plant infection mechanisms by a symbiont and a pathogen.

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