These authors contributed equally to this work.
The Arabidopsis J-protein AtDjB1 facilitates thermotolerance by protecting cells against heat-induced oxidative damage
Article first published online: 22 FEB 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 194, Issue 2, pages 364–378, April 2012
How to Cite
Zhou, W., Zhou, T., Li, M.-X., Zhao, C.-L., Jia, N., Wang, X.-X., Sun, Y.-Z., Li, G.-L., Xu, M., Zhou, R.-G. and Li, B. (2012), The Arabidopsis J-protein AtDjB1 facilitates thermotolerance by protecting cells against heat-induced oxidative damage. New Phytologist, 194: 364–378. doi: 10.1111/j.1469-8137.2012.04070.x
- Issue published online: 19 MAR 2012
- Article first published online: 22 FEB 2012
- Received: 24 October 2011, Accepted: 8 January 2012
- ascorbate (ASC);
- hydrogen peroxide (H2O2);
- oxidative damage;
- •AtDjB1 belongs to the J-protein family in Arabidopsis thaliana. Its biological functions in plants are largely unknown.
- •In this study, we examined the roles of AtDjB1 in resisting heat and oxidative stresses in A. thaliana using reverse genetic analysis.
- •AtDjB1 knockout plants (atj1-1) were more sensitive to heat stress than wildtype plants, and displayed decreased concentrations of ascorbate (ASC), and increased concentrations of hydrogen peroxide (H2O2) and oxidative products after heat shock. Application of H2O2 accelerated cell death and decreased seedling viability in atj1-1. Exogenous ASC conferred much greater thermotolerance in atj1-1 than in wildtype plants, suggesting that a lower concentration of ASC in atj1-1 could be responsible for the increased concentration of H2O2 and decreased thermotolerance. Furthermore, AtDjB1 was found to localize to mitochondria, directly interact with a mitochondrial heat-shock protein 70 (mtHSC70-1), and stimulate ATPase activity of mtHSC70-1. AtDjB1 knockout led to the accumulation of cellular ATP and decreased seedling respiration, indicating that AtDjB1 modulated the ASC concentration probably through affecting the function of mitochondria.
- •Taken together, these results suggest that AtDjB1 plays a crucial role in maintaining redox homeostasis, and facilitates thermotolerance by protecting cells against heat-induced oxidative damage.