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Fig. S1 Beet curly top virus (BCTV) DNA accumulation is not affected by co-infection with Tomato yellow leaf curl Sardinia virus (TYLCSV) or mutations in C2, C4 or V2 genes

Fig. S2 Rep-dependent green fluorescent protein (GFP) expression and mGFP production are enhanced by Beet curly top virus (BCTV) C2 but not by TYLCSV C2.

Fig. S3 Beet curly top virus (BCTV) Rep-dependent green fluorescent protein (GFP) expression and mGFP production from BTCV 2IR constructs are enhanced by C2, but not by C4 or V2.

Fig. S4 Beet curly top virus (BCTV) C2 does not enhance the accumulation of RNA viruses.

Fig. S5 Expression levels of cell cycle and DNA packaging genes in Arabidopsis transgenic plants expressing Beet curly top virus (BCTV) C2 or Tomato yellow leaf curl Sardinia virus (TYLCSV) C2.

Fig. S6 Beet curly top virus (BCTV) C2 induces the expression of CDKB2, CYCA1 and CYCA2 in Nicotiana benthamiana.

Fig. S7 Wild-type Beet curly top virus (BCTV) C2 and C2-2 mutant, but not C2-3 mutant, induce the expression of CDKB2, CYCA1 and CYCA2 in transgenic 2IR-GFP Nicotiana benthamiana plants.

Table S1 Oligonucleotides used in this work

Table S2 Primer combinations used to amplify plant or viral genes

Table S3 Probes used in this work

Methods S1 Additional information for experimental procedures.

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