The plant-specific G protein γ subunit AGG3 influences organ size and shape in Arabidopsis thaliana

Authors

  • Shengjun Li,

    1. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
    2. Graduate School, Chinese Academy of Sciences, Beijing 100039, China
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    • These authors contributed equally to this work.

  • Yaju Liu,

    1. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
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    • These authors contributed equally to this work.

  • Leiying Zheng,

    1. Centre of Bioenergy, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
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  • Liangliang Chen,

    1. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
    2. Graduate School, Chinese Academy of Sciences, Beijing 100039, China
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  • Na Li,

    1. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
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  • Fiona Corke,

    1. Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, UK
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  • Yaru Lu,

    1. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
    2. College of Biological Sciences, Hebei Normal University, Shijiazhuang, China
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  • Xiangdong Fu,

    1. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
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  • Zhengge Zhu,

    1. College of Biological Sciences, Hebei Normal University, Shijiazhuang, China
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  • Michael W. Bevan,

    1. Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, UK
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  • Yunhai Li

    1. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
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Author for correspondence:
Yunhai Li
Tel: +86 10 64807856
Email: yhli@genetics.ac.cn

Summary

  • Control of organ size and shape by cell proliferation and cell expansion is a fundamental developmental process, but the mechanisms that set the size and shape of determinate organs are largely unknown in plants.
  • Molecular, genetic, cytological and biochemical approaches were used to characterize the roles of the Arabidopsis thaliana G protein γ subunit (AGG3) gene in organ growth.
  • Here, we describe A. thaliana AGG3, which promotes petal growth by increasing the period of cell proliferation. Both the N-terminal region and the C-terminal domains of AGG3 are necessary for the function of AGG3. By contrast, analysis of a series of AGG3 derivatives with deletions in specific domains showed that the deletion of any of these domains cannot completely abolish the function of AGG3. The GFP-AGG3 fusion protein is localized to the plasma membrane. The predicted transmembrane domain plays an important role in the plasma membrane localization of AGG3. Genetic analyses revealed that AGG3 action requires a functional G protein α subunit (GPA1) and G protein β subunit (AGB1).
  • Our findings demonstrate that AGG3, GPA1 and AGB1 act in the same genetic pathway to influence organ size and shape in A. thaliana.

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