These authors contributed equally to this work.
The plant-specific G protein γ subunit AGG3 influences organ size and shape in Arabidopsis thaliana
Article first published online: 1 MAR 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 194, Issue 3, pages 690–703, May 2012
How to Cite
Li, S., Liu, Y., Zheng, L., Chen, L., Li, N., Corke, F., Lu, Y., Fu, X., Zhu, Z., Bevan, M. W. and Li, Y. (2012), The plant-specific G protein γ subunit AGG3 influences organ size and shape in Arabidopsis thaliana. New Phytologist, 194: 690–703. doi: 10.1111/j.1469-8137.2012.04083.x
- Issue published online: 10 APR 2012
- Article first published online: 1 MAR 2012
- Received: 9 November 2011, Accepted: 22 January 2012
Fig. S1 SHORT ORGAN 1 (STN1) increases cell proliferation.
Fig. S2 Molecular characterization of the SHORT ORGAN 1 (STN1) gene.
Fig. S3 Alignment of the Arabidopsis thaliana G protein γ subunit (AGG3) sequence with the Brassica rapa BrAGG3 protein.
Fig. S4 Phylogenetic tree of G protein γ subunit (AGG3) homologs.
Fig. S5 Alignment of the Arabidopsis thaliana G protein γ subunit (AGG3) sequence with rice (Oryza sativa) GRAIN SIZE 3 (GS3) and DENSE AND ERECT PANICLE1 (DEP1) PANICLE ERECTNESS (qPE9-1) proteins.
Fig. S6 Expression levels of Arabidopsis thaliana G protein γ subunit (AGG3) and BrAGG3 in transgenic plants.
Fig. S7 GUS activity of ProAGG3:GUS and ProCYCB1; 1:CDB-GUS petals.
Fig. S8 GFP fluorescence in GFP-AGG3 and GFP-AGG3Δ108−125 lines.
Fig. S9 GFP-AGG3Δ108−125 proteins were detected in the membrane fraction.
Fig. S10 Molecular characterization of gpa1-101 and agb1-2 mutants.
Fig. S11 Overexpression of G protein β subunit (AGB1) or G protein α subunit (GPA1) could not complement the petal size phenotype of agg3-2.
Fig. S12 Increased organ size in 35S:GFP-AGG3 is dependent on functional G protein β subunit (AGB1) and G protein α subunit (GPA1).
Table S1 List of primers used in this study
Table S2 Phenotyes of wild-type and agg3 plants
Methods S1 Morphological and cellular analyses.
Methods S2 GUS staining.
Methods S3 Protein extraction and western blot.
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