Autophagy machinery controls nitrogen remobilization at the whole-plant level under both limiting and ample nitrate conditions in Arabidopsis

Authors

  • Anne Guiboileau,

    1. INRA, UMR1318, Institut Jean-Pierre Bourgin, RD10, F-78000 Versailles, France
    2. AgroParisTech, Institut Jean-Pierre Bourgin, RD10, F-78000 Versailles, France
    Search for more papers by this author
  • Kohki Yoshimoto,

    1. INRA, UMR1318, Institut Jean-Pierre Bourgin, RD10, F-78000 Versailles, France
    2. AgroParisTech, Institut Jean-Pierre Bourgin, RD10, F-78000 Versailles, France
    3. RIKEN, Plant Science Center, Tsurumi-ku, Yokohama 230-0045, Japan
    Search for more papers by this author
  • Fabienne Soulay,

    1. INRA, UMR1318, Institut Jean-Pierre Bourgin, RD10, F-78000 Versailles, France
    2. AgroParisTech, Institut Jean-Pierre Bourgin, RD10, F-78000 Versailles, France
    Search for more papers by this author
  • Marie-Paule Bataillé,

    1. INRA, UMR INRA-UCBN 950, Ecophysiologie Végétale, Agronomie et Nutritions N, C, S, Université de Caen, 14000 Caen, France
    Search for more papers by this author
  • Jean-Christophe Avice,

    1. INRA, UMR INRA-UCBN 950, Ecophysiologie Végétale, Agronomie et Nutritions N, C, S, Université de Caen, 14000 Caen, France
    Search for more papers by this author
  • Céline Masclaux-Daubresse

    1. INRA, UMR1318, Institut Jean-Pierre Bourgin, RD10, F-78000 Versailles, France
    2. AgroParisTech, Institut Jean-Pierre Bourgin, RD10, F-78000 Versailles, France
    Search for more papers by this author

Author for correspondence:
Céline Masclaux-Daubresse
Tel: +33 1 30 83 30 88
Email: celine.masclaux@versailles.inra.fr

Summary

  • Processes allowing the recycling of organic nitrogen and export to young leaves and seeds are important determinants of plant yield, especially when plants are nitrate-limited. Because autophagy is induced during leaf ageing and in response to nitrogen starvation, its role in nitrogen remobilization was suspected. It was recently shown that autophagy participates in the trafficking of Rubisco-containing bodies to the vacuole.
  • To investigate the role of autophagy in nitrogen remobilization, several autophagy-defective (atg) Arabidopsis mutants were grown under low and high nitrate supplies and labeled with inline image at the vegetative stage in order to determine 15N partitioning in seeds at harvest. Because atg mutants displayed earlier and more rapid leaf senescence than wild type, we investigated whether their defects in nitrogen remobilization were related to premature leaf cell death by studying the stay-green atg5.sid2 and atg5.NahG mutants.
  • Results showed that nitrogen remobilization efficiency was significantly lower in all the atg mutants irrespective of biomass defects, harvest index reduction, leaf senescence phenotypes and nitrogen conditions.
  • We conclude that autophagy core machinery is needed for nitrogen remobilization and seed filling.

Ancillary