Fig. S1 Bright field (left) and confocal fluorescence images (right) of protoplasts transfected with NitRate Transporter families NRT2.2-2.7 genes fused to cEYFP and ABCG12fused to nEYFP used as negative control.

Table S1 Oligonucleotide primer sequences with gene-specific sequences highlighted in gray used for cloning into Y-2-H bait and prey vectors

Table S2 Oligonucleotide primer sequences containing attB1/attB2 Gateway® recombination sites and gene-specific sequences highlighted in gray, used for cloning into Gateway® pDONR221 donor vector

Table S3 Oligonucleotide primer sequences with gene-specific sequences underlined, used for cloning into split-YFP expression vectors

Table S4 15NO3- uptake (delta 15N) by Xenopus oocytes following single injections with water, cNAR2.1 (Nitrate Assimilation Related family), or cNRT2s (NitRate Transporter families), and following co-injections of NRT2s together with AtNAR2.1

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