These authors contributed equally to this work.
Biological control of bacterial wilt in Arabidopsis thaliana involves abscissic acid signalling
Article first published online: 20 MAR 2012
© 2012 INRA. New Phytologist © 2012 New Phytologist Trust
Volume 194, Issue 4, pages 1035–1045, June 2012
How to Cite
Feng, D. X., Tasset, C., Hanemian, M., Barlet, X., Hu, J., Trémousaygue, D., Deslandes, L. and Marco, Y. (2012), Biological control of bacterial wilt in Arabidopsis thaliana involves abscissic acid signalling. New Phytologist, 194: 1035–1045. doi: 10.1111/j.1469-8137.2012.04113.x
- Issue published online: 2 MAY 2012
- Article first published online: 20 MAR 2012
- Received: 13 October 2011, Accepted: 8 February 2012
- abscissic acid (ABA);
- Arabidopsis thaliana;
- biological control;
- defense signalling;
- hrp genes;
- Ralstonia solanacearum
- •Means to control bacterial wilt caused by the phytopathogenic root bacteria Ralstonia solanacearum are limited. Mutants in a large cluster of genes (hrp) involved in the pathogenicity of R. solanacearum were successfully used in a previous study as endophytic biocontrol agents in challenge inoculation experiments on tomato. However, the molecular mechanisms controlling this resistance remained unknown.
- •We developed a protection assay using Arabidopsis thaliana as a model plant and analyzed the events underlying the biological control by genetic, transcriptomic and molecular approaches.
- •High protection rates associated with a significant decrease in the multiplication of R. solanacearum were observed in plants pre-inoculated with a ΔhrpB mutant strain. Neither salicylic acid, nor jasmonic acid/ethylene played a role in the establishment of this resistance. Microarray analysis showed that 26% of the up-regulated genes in protected plants are involved in the biosynthesis and signalling of abscissic acid (ABA). In addition 21% of these genes are constitutively expressed in the irregular xylem cellulose synthase mutants (irx), which present a high level of resistance to R. solanacearum.
- •We propose that inoculation with the ΔhrpB mutant strain generates a hostile environment for subsequent plant colonization by a virulent strain of R. solanacearum.