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Fig. S1 DNA sequencer gel electrophoresis of the SSR locus shows that PF and PS had different SSR genotypes, ‘290/290’ and ‘289/289’, respectively, indicating that PF and PS belong to different conspecific genets.

Fig. S2 Time-course autoradiograms (0, 1 and 3 d after 14CO2 feeding) of 14C distribution in a rhizobox of paired PF-colonised Pinus thunbergii seedlings.

Fig. S3 Scanned photograph (left) and autoradiogram (right, 14 d after 14CO2 feeding) of a rhizobox in which PF- and PS-colonised Pinus thunbergii seedlings were paired.

Fig. S4 Time-course autoradiograms (0, 3, 7 and 14 d after 33P feeding) of 33P distribution in a rhizobox of PF-colonised Pinus thunbergii seedlings.

Fig. S5 Scanned photograph (left) and 33P autoradiogram (right, 14 d after 33P feeding) of a rhizobox in which PF- and PS-colonised Pinus thunbergii seedlings were paired.

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