Loss of cytosolic NADP-malic enzyme 2 in Arabidopsis thaliana is associated with enhanced susceptibility to Colletotrichum higginsianum

Authors

  • Lars M. Voll,

    1. Division of Biochemistry, Department of Biology, Friedrich-Alexander-University Erlangen-Nuremberg, Staudtstr. 5, 91058 Erlangen, Germany
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    • These authors contributed equally to this work.

  • Martina B. Zell,

    1. Institute for Developmental and Molecular Biology of Plants, Heinrich-Heine-University, Universitätsstr. 1, 40225 Düsseldorf, Germany
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    • These authors contributed equally to this work.

  • Timo Engelsdorf,

    1. Division of Biochemistry, Department of Biology, Friedrich-Alexander-University Erlangen-Nuremberg, Staudtstr. 5, 91058 Erlangen, Germany
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  • Alexandra Saur,

    1. Division of Biochemistry, Department of Biology, Friedrich-Alexander-University Erlangen-Nuremberg, Staudtstr. 5, 91058 Erlangen, Germany
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  • Mariel Gerrard Wheeler,

    1. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Universidad Nacional de Rosario, Suipacha 531, Rosario, Argentina
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  • Maria F. Drincovich,

    1. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Universidad Nacional de Rosario, Suipacha 531, Rosario, Argentina
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  • Andreas P. M. Weber,

    1. Institute of Biochemistry, Heinrich-Heine-University, Universitätsstr. 1, 40225 Düsseldorf, Germany
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  • Veronica G. Maurino

    1. Institute for Developmental and Molecular Biology of Plants, Heinrich-Heine-University, Universitätsstr. 1, 40225 Düsseldorf, Germany
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Author for correspondence:
Veronica G. Maurino
Tel: +49 211 8115446
Email: veronica.maurino@uni-duesseldorf.de

Summary

  • While photosynthetic NADP-malic enzyme (NADP-ME) has a prominent role in the C4 cycle, the biological function of nonphotosynthetic isoforms remains elusive. Here, we analysed the link between Arabidopsis thaliana cytosolic NADP-ME2 and the plant defence response.
  • Arabidopsis thaliana plants with wild-type and modified NADP-ME2 expression levels were analysed after elicitation with pathogen-associated molecular patterns (PAMPs) and during the interaction with the hemibiotrophic fungal pathogen Colletotrichum higginsianum.
  • Under normal growth conditions, the lack or gain of NADP-ME2 activity produced large changes in plant metabolite pool sizes without any effect on morphology or development. Total NADP-ME activity and NADP-ME2 transcript level were enhanced after PAMP treatment and pathogen infection. During infection with C. higginsianum, loss-of-function mutants of NADP-ME2 (nadp-me2) showed enhanced susceptibility. Transient apoplastic reactive oxygen species (ROS) production after elicitation and callose papilla formation after infection were dampened in nadp-me2. Late salicylic acid (SA)-dependent and SA-independent defence responses were not affected.
  • Taken together, our results indicate that NADP-ME2 is an important player in plant basal defence, where it appears to be involved in the generation of ROS. Moreover, NADP-ME2 was found to be dispensable for later defence responses.

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