These authors contributed equally to this work.
Loss of cytosolic NADP-malic enzyme 2 in Arabidopsis thaliana is associated with enhanced susceptibility to Colletotrichum higginsianum
Article first published online: 12 APR 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 195, Issue 1, pages 189–202, July 2012
How to Cite
Voll, L. M., Zell, M. B., Engelsdorf, T., Saur, A., Wheeler, M. G., Drincovich, M. F., Weber, A. P. M. and Maurino, V. G. (2012), Loss of cytosolic NADP-malic enzyme 2 in Arabidopsis thaliana is associated with enhanced susceptibility to Colletotrichum higginsianum. New Phytologist, 195: 189–202. doi: 10.1111/j.1469-8137.2012.04129.x
- Issue published online: 24 MAY 2012
- Article first published online: 12 APR 2012
- Received: 19 December 2011, Accepted: 22 February 2012
- Colletotrichum higginsianum;
- NADP-malic enzyme (NADP-ME);
- pathogen-associated molecular pattern (PAMP);
- reactive oxygen species (ROS)
- •While photosynthetic NADP-malic enzyme (NADP-ME) has a prominent role in the C4 cycle, the biological function of nonphotosynthetic isoforms remains elusive. Here, we analysed the link between Arabidopsis thaliana cytosolic NADP-ME2 and the plant defence response.
- •Arabidopsis thaliana plants with wild-type and modified NADP-ME2 expression levels were analysed after elicitation with pathogen-associated molecular patterns (PAMPs) and during the interaction with the hemibiotrophic fungal pathogen Colletotrichum higginsianum.
- •Under normal growth conditions, the lack or gain of NADP-ME2 activity produced large changes in plant metabolite pool sizes without any effect on morphology or development. Total NADP-ME activity and NADP-ME2 transcript level were enhanced after PAMP treatment and pathogen infection. During infection with C. higginsianum, loss-of-function mutants of NADP-ME2 (nadp-me2) showed enhanced susceptibility. Transient apoplastic reactive oxygen species (ROS) production after elicitation and callose papilla formation after infection were dampened in nadp-me2. Late salicylic acid (SA)-dependent and SA-independent defence responses were not affected.
- •Taken together, our results indicate that NADP-ME2 is an important player in plant basal defence, where it appears to be involved in the generation of ROS. Moreover, NADP-ME2 was found to be dispensable for later defence responses.