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Fig. S1 Quantitative real-time PCR analysis of NcZNT1 expression in roots and shoots of N.  caerulescens plants grown on varying Zn levels (0–50 μM Zn) for 7 d.

Fig. S2 Quantitative real-time PCR analysis of NcZNT1 expression in roots and shoots of N.  caerulescens plants in response to growth on nutrient solution.

Fig. S3 Complementation of yeast mutants defective in the uptake of Cu (∆ctr1/ctr3), Fe (∆fet3/fet4), Zn (∆zrt1/zrt2) or Mn (∆smf1), expressing NcZNT1 or the empty vector, pFL61.

Fig. S4 Western blot analysis of NcZNT1 with the microsomal fraction of N. caerulescens roots. SDS-PAGE and Western blot analyses were performed using the anti-NcZNT1 antibody.

Fig. S5 Representative plants of either Col-0 or the NcZNT1-1 overexpression line, grown on nutrient solutions.

Fig. S6 Transgenic plants expressing the full length (1.1 kb) NcZNT1p::GUS construct grown on a range of Zn concentrations and stained for GUS activity.

Fig. S7 Sequence of the c. 1.1 kb promoter region of NcZNT1 with the putative hyperexpression region and the Zn-responsive elements highlighted.

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