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Fig. S1 Significant correlations between parameters of plant N acquisition, plant biomass, and nodule traits for plants grown under hydro-aeroponic conditions.

Fig. S2 Effect of the bacterial strain on shoot N content in N2-fixing plants submitted to short term local N limitation.

Fig. S3 Split-roots systems.

Fig. S4 Distribution of small and bigger nodules on roots of plants co-infected with RCR2011 and its Fix mutant strain.

Table S1 Effect of the rhizobial strain on nodule activity, plant N acquisition, biomass partitioning and nodule traits for plants grown under hydro-aeroponic conditions

Table S2 Effect of the rhizobial strain on plant N acquisition, plant biomass partitioning and nodule traits

Table S3 Effect of the Fix+ bacterial strain on responses (N acquisition and plant biomass) of N2-fixing plants to long term local N limitation

Table S4 Effect of the Fix+ bacterial strain in mixed nodule populations on responses (N acquisition and plant biomass) of N2-fixing plants to long-term local N limitation

Table S5 Effect of homogenous co-infection by md4 and RCR2011 of the whole root system on plant and nodule development

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