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Fig. S1 The hydroponic split-root culture system used for nitrogen (N) deficiency-induced and dark-induced senescence.

Fig. S2 The effect of N and Fe treatments on zinc (Zn) (a), copper (Cu) (b) and manganese (Mn) (c) concentrations in different barley organs at final harvest.

Fig. S3 The effect of N and Fe treatments on concentrations of iron (Fe)-citrate complexes in old barley leaves.

Fig. S4 The effect of shading treatment on zinc (Zn) (a), copper (Cu) (b) and manganese (Mn) (c) concentrations in different barley organs at final harvest.

Fig. S5 The effect of shading treatment on concentrations of Fe-citrate complexes in old barley leaves.

Table S1 Iron (Fe) contents in different barley organs at two harvest time-points under different nitrogen (N) and Fe treatments

Table S2 Iron (Fe) uptake of barley plants under different nitrogen (N) and Fe treatments

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