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Fig. S1 Confocal microscope images of Wt tobacco mesophyll cells, showing the lack of chlorophyll fluorescence signal in the 500–530 and 585–615 nm ranges.

Fig. S2 Confocal microscope images of mitochondrial O2- in Wt tobacco mesophyll cells either left untreated or treated with antimycin A and/or SOD-PEG.

Fig. S3 Confocal microscope images of Wt tobacco mesophyll cells, showing the comparable number of mitochondrial signals generated by MitoSOX Red and MitoTracker Red.

Fig. S4 Confocal microscope images of cellular NO in Wt tobacco mesophyll cells either left untreated or treated with sodium nitroprusside and/or cPTIO.

Fig. S5 Confocal microscope images of cellular ONOO- in Wt tobacco mesophyll cells either left untreated or treated with SIN1 and/or Ebselen.

Fig. S6 Confocal microscope images of cellular ONOO- in Wt and transgenic plants with altered AOX concentrations.

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