The Pht1;9 and Pht1;8 transporters mediate inorganic phosphate acquisition by the Arabidopsis thaliana root during phosphorus starvation
Version of Record online: 11 MAY 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 195, Issue 2, pages 356–371, July 2012
How to Cite
Remy, E., Cabrito, T. R., Batista, R. A., Teixeira, M. C., Sá-Correia, I. and Duque, P. (2012), The Pht1;9 and Pht1;8 transporters mediate inorganic phosphate acquisition by the Arabidopsis thaliana root during phosphorus starvation. New Phytologist, 195: 356–371. doi: 10.1111/j.1469-8137.2012.04167.x
- Issue online: 15 JUN 2012
- Version of Record online: 11 MAY 2012
- Received: 16 January 2012, Accepted: 4 April 2012
- 1994. Transient transformation of Arabidopsis leaf protoplasts: a versatile experimental system to study gene expression. Plant Journal 5: 421–427. , .
- 1966. Assay of inorganic phosphate, total phosphate and phosphatases. Methods in Enzymology 8: 115–118. .
- 2006. pho2, a phosphate overaccumulator, is caused by a nonsense mutation in a microRNA399 target gene. Plant Physiology 141: 1000–1011. , , , , , .
- 1996. Stimulation of root hair elongation in Arabidopsis thaliana by low phosphate availability. Plant, Cell & Environment 19: 529–538. , .
- 2011. Arabidopsis thaliana high-affinity phosphate transporters exhibit multiple levels of posttranslational regulation. Plant Cell 23: 1523–1535. , , , , , , , , .
- 1973. Phosphate pools, phosphate transport, and phosphate availability. Annual Review of Plant Physiology and Plant Molecular Biology 24: 225–252. .
- 1991. The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter. Molecular & Cell Biology 11: 3229–3238. , , , .
- 2010. A central regulatory system largely controls transcriptional activation and repression responses to phosphate starvation in Arabidopsis. PLoS Genetics 6: e1001102. , , , , , , , , .
- 2009. Heterologous expression of a Tpo1 homolog from Arabidopsis thaliana confers resistance to the herbicide 2,4-d and other chemical stresses in yeast. Applied Microbiology and Biotechnology 84: 927–936. , , , , .
- 2007. A mutant of the Arabidopsis phosphate transporter PHT1;1 displays enhanced arsenic accumulation. Plant Cell 19: 1123–1133. , , , , , , , .
- 2006. Regulation of phosphate homeostasis by microRNA in Arabidopsis. Plant Cell 18: 412–421. , , , , , .
- 1998. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant Journal 16: 735–743. , .
- 2009. The phosphate transporter PHT4;6 is a determinant of salt tolerance that is localized to the Golgi apparatus of Arabidopsis. Molecular Plant 2: 535–552. , , , , , , , , .
- 1998. Functional analysis and cell-specific expression of a phosphate transporter from tomato. Planta 206: 225–233. , , , , .
- 1999. Pht2;1 encodes a low-affinity phosphate transporter from Arabidopsis. Plant Cell 11: 2153–2166. , , , , , .
- 1995. Characterization of a phosphate-accumulator mutant of Arabidopsis thaliana. Plant Physiology 107: 207–213. , .
- 2004. Characterization of anion channels in the plasma membrane of Arabidopsis epidermal root cells and the identification of a citrate-permeable channel induced by phosphate starvation. Plant Physiology 136: 4136–4149. , , , .
- Eaton AD, Clesceri LS, Rice EW, Greenberg AE, eds. 2005. Standard methods for the examination of water and wastewater. 21st ed . Washington, DC, USA: American Public Health Association, American Water Works Association, Water Environment Federation.
- 2005. PHOSPHATE TRANSPORTER TRAFFIC FACILITATOR1 is a plant-specific SEC12-related protein that enables the endoplasmic reticulum exit of a high-affinity phosphate transporter in Arabidopsis. Plant Cell 17: 3500–3512. , , , , .
- 2006. Transcription analysis of Arabidopsis membrane transporters and hormone pathways during developmental and induced leaf senescence. Plant Physiology 141: 776–792. , , , , , .
- 2008. Functional analysis of the Arabidopsis PHT4 family of intracellular phosphate transporters. New Phytologist 177: 889–898. , , , , , .
- 2003. A chemical-regulated inducible RNAi system in plants. Plant Journal 34: 383–392. , , , .
- 2000. Characterization of Arabidopsis acid phosphatase promoter and regulation of acid phosphatase expression. Plant Physiology 124: 615–626. , , , , .
- 2006. How do plants respond to nutrient shortage by biomass allocation? Trends in Plant Science 11: 610–617. , , , .
- 2006. Phosphate deficiency promotes modification of iron distribution in Arabidopsis plants. Biochimie 88: 1767–1771. , , , , , , .
- 2009. Variations in the composition of gelling agents affect morphophysiological and molecular responses to deficiencies of phosphate and other nutrients. Plant Physiology 150: 1033–1049. , , , , , , .
- 2005. Drag & drop cloning in yeast. Gene 344: 43–51. , , , , .
- 2005. Symbiotic phosphate transport in arbuscular mycorrhizas. Trends in Plant Science 10: 22–29. , .
- 2009. Promoter deletion analysis elucidates the role of cis elements and 5’UTR intron in spatiotemporal regulation of AtPht1;4 expression in Arabidopsis. Physiologia Plantarum 136: 10–18. , , .
- 2002. Regulated expression of Arabidopsis phosphate transporters. Plant Physiology 130: 221–233. , , , , , .
- 2003. Analysis of the plastidic phosphate translocator gene family in Arabidopsis and identification of new phosphate translocator-homologous transporters, classified by their putative substrate-binding site. Plant Physiology 131: 1178–1190. , , .
- 1997. Two cDNAs from potato are able to complement a phosphate uptake-deficient yeast mutant: identification of phosphate transporters from higher plants. Plant Cell 9: 381–392. , , .
- 2008. Regulatory network of microRNA399 and PHO2 by systemic signaling. Plant Physiology 147: 732–746. , , , , , , .
- 2009. Molecular regulators of phosphate homeostasis in plants. Journal of Experimental Botany 60: 1427–1438. , , .
- 2002. Nitrate and phosphate availability and distribution have different effects on root system architecture of Arabidopsis. Plant Journal 29: 751–760. , , , .
- 2008. Closely related members of the Medicago truncatula PHT1 phosphate transporter gene family encode phosphate transporters with distinct biochemical activities. Journal of Biological Chemistry 283: 24673–24681. , , , , , .
- 2003. The role of nutrient availability in regulating root architecture. Current Opinion in Plant Biology 6: 280–287. , , .
- 2000. Enhanced phosphorus uptake in transgenic tobacco plants that overproduce citrate. Nature Biotechnology 18: 450–453. , , , .
- 2002. Phosphate availability alters architecture and causes changes in hormone sensitivity in the Arabidopsis root system. Plant Physiology 129: 244–256. , , , , , .
- 1995. Mineral nutrition of higher plants. London, UK: Academic Press. .
- 1998. Identification, cloning and characterization of a derepressible Na+-coupled phosphate transporter in Saccharomyces cerevisiae. Molecular & General Genetics 258: 628–638. , .
- 1992. Suppression of the high-affinity phosphate-uptake system – a mechanism of arsenate tolerance in Holcus-lanatus l. Journal of Experimental Botany 43: 519–524. , .
- 1990. Phosphate-transport across biomembranes and cytosolic phosphate homeostasis in barley leaves. Planta 180: 139–146. , , , , , .
- 2005. A genome-wide transcriptional analysis using Arabidopsis thaliana Affymetrix gene chips determined plant responses to phosphate deprivation. Proceedings of the National Academy of Sciences, USA 102: 11934–11939. , , , , , , , , , et al.
- 2004. Transcriptional regulation and functional properties of Arabidopsis Pht1;4, a high affinity transporter contributing greatly to phosphate uptake in phosphate deprived plants. Plant Molecular Biology 55: 727–741. , , , , .
- 1997. Overexpression of an Arabidopsis thaliana high-affinity phosphate transporter gene in tobacco cultured cells enhances cell growth under phosphate-limited conditions. Proceedings of the National Academy of Sciences, USA 94: 7098–7102. , , , , , , .
- 2007. Genome-wide reprogramming of metabolism and regulatory networks of Arabidopsis in response to phosphorus. Plant, Cell & Environment 30: 85–112. , , , , , , , , , et al.
- 1996. Phosphate transporters from the higher plant Arabidopsis thaliana. Proceedings of the National Academy of Sciences, USA 93: 10519–10523. , , .
- 2002. Expression analysis suggests novel roles for members of the Pht1 family of phosphate transporters in Arabidopsis. Plant Journal 31: 341–353. , , , .
- 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 15: 473–497. , .
- 2011. Arabidopsis Pht1;5 mobilizes phosphate between source and sink organs and influences the interaction between phosphate homeostasis and ethylene signaling. Plant Physiology 156: 1149–1163. , , , , , .
- 2011. Phosphate import in plants: focus on the Pht1 transporters. Frontiers in Plant Science 2: 1–12. , , , , , , , .
- 2008. MicroRNA399 is a long-distance signal for the regulation of plant phosphate homeostasis. Plant Journal 53: 731–738. , , , .
- 1998. Major facilitator superfamily. Microbiology & Molecular Biology Reviews 62: 1–34. , , .
- 2011. Root developmental adaptation to phosphate starvation: better safe than sorry. Trends in Plant Science 16: 442–450. , , , .
- 2008. Phosphate availability alters lateral root development in Arabidopsis by modulating auxin sensitivity via a mechanism involving the TIR1 auxin receptor. Plant Cell 20: 3258–3272. , , , , , , .
- 1991. Mutant of Arabidopsis deficient in xylem loading of phosphate. Plant Physiology 97: 1087–1093. , , , .
- 1999. A type 5 acid phosphatase gene from Arabidopsis thaliana is induced by phosphate starvation and by some other types of phosphate mobilising/oxidative stress conditions. Plant Journal 19: 579–589. , , , , , , .
- 2003. Characterization of two phosphate transporters from barley; evidence for diverse function and kinetic properties among members of the Pht1 family. Plant Molecular Biology 53: 27–36. , , , .
- 1999. Phosphate acquisition. Annual Review of Plant Physiology & Plant Molecular Biology 50: 665–693. .
- 2002. Molecular mechanisms of phosphate transport in plants. Planta 216: 23–37. , .
- 2010. Regulation of phosphate starvation responses in plants: signaling players and cross-talks. Molecular Plant 3: 288–299. , , .
- 2001. A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae. Genes & Development 15: 2122–2133. , , , , , , .
- 2005. Phosphate starvation induces a determinate developmental program in the roots of Arabidopsis thaliana. Plant & Cell Physiology 46: 174–184. , , , , , , .
- 2006. Characterization of low phosphorus insensitive mutants reveals a crosstalk between low phosphorus-induced determinate root development and the activation of genes involved in the adaptation of Arabidopsis to phosphorus deficiency. Plant Physiology 140: 879–889. , , , , , .
- 1998. Phosphorus uptake by plants: from soil to cell. Plant Physiology 116: 447–453. , , .
- 1978. Characterization of the plasma-membrane ATPase of Saccharomyces-cerevisiae. Molecular & Cellular Biochemistry 22: 51–63. .
- 2004. Phosphate transport in Arabidopsis: Pht1;1 and Pht1;4 play a major role in phosphate acquisition from both low- and high-phosphate environments. Plant Journal 39: 629–642. , , , .
- 2003. Phosphate transport in plants. Plant and Soil 248: 71–83. , , , .
- 2007. Root tip contact with low-phosphate media reprograms plant root architecture. Nature Genetics 39: 792–796. , , , , , , , .
- 1999. Isolation and characterization of cDNAs encoding mitochondrial phosphate transporters in soybean, maize, rice, and Arabidopsis. Plant Molecular Biology 40: 479–486. , , , , , .
- 2010. Dissection of local and systemic transcriptional responses to phosphate starvation in Arabidopsis. Plant Journal 64: 775–789. , , , , , , , , .
- 2004. Arabidopsis pdr2 reveals a phosphate-sensitive checkpoint in root development. Plant Journal 37: 801–814. , , , , .
- 2007. Isolation and comparative analysis of the wheat TaPT2 promoter: identification in silico of new putative regulatory motifs conserved between monocots and dicots. Journal of Experimental Botany 58: 2573–2582. , , , , , , , , , et al.
- 2007. Saccharomyces cerevisiae multidrug resistance transporter Qdr2 is implicated in potassium uptake, providing a physiological advantage to quinidine-stressed cells. Eukaryotic Cell 6: 134–142. , , , , , , .
- 2002. A chloroplast phosphate transporter, PHT2;1, influences allocation of phosphate within the plant and phosphate-starvation responses. Plant Cell 14: 1751–1766. , .
- 1995. Repressible cation-phosphate symporters in Neurospora crassa. Proceedings of the National Academy of Sciences, USA 92: 3884–3887. , .
- 2008. The effect of iron on the primary root elongation of Arabidopsis during phosphate deficiency. Plant Physiology 147: 1181–1191. , , , , .
- 2008. Quantitative trait locus mapping for seed mineral concentrations in two Arabidopsis thaliana recombinant inbred populations. New Phytologist 179: 1033–1047. , .
- 2001. Phosphate availability regulates root system architecture in Arabidopsis. Plant Physiology 126: 875–882. , , , .
- 2007. Positive feedback regulates switching of phosphate transporters in S. cerevisiae. Molecular Cell 27: 1005–1013. , , , , .
- 2007. Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nature Protocols 2: 1565–1572. , , .
- 2009. Physiological and transcriptome analysis of iron and phosphorus interaction in rice seedlings. Plant Physiology 151: 262–274. , , , , , , , , , et al.
- 2004. Genevestigator. Arabidopsis microarray database and analysis toolbox. Plant Physiology 136: 2621–2632. , , , .